| Title: | Differential Requirement for the Cell Wall Integrity Sensor Wsc1p in Diploids Versus Haploids |
| Author(s): | Hall AE; Lisci M; Rose MD; |
| Address: | "Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. Helen L. and Martin S. Kimmel Center for Biology and Medicine, Skirball Institute of Biomolecular Medicine, New York, NY 10016, USA. Cambridge Institute for Medical Research, University of Cambridge, Cambridge Biomedical Campus, Cambridge CB2 0XY, UK. Department of Biology, Georgetown University, Washington, DC 20057, USA" |
| ISSN/ISBN: | 2309-608X (Electronic) 2309-608X (Linking) |
| Abstract: | "The primary role of the Cell Wall Integrity Pathway (CWI) in Saccharomyces cerevisiae is monitoring the state of the cell wall in response to general life cycle stresses (growth and mating) and imposed stresses (temperature changes and chemicals). Of the five mechanosensor proteins monitoring cell wall stress, Wsc1p and Mid2p are the most important. We find that WSC1 has a stringent requirement in zygotes and diploids, unlike haploids, and differing from MID2's role in shmoos. Diploids lacking WSC1 die frequently, independent of mating type. Death is due to loss of cell wall and plasma membrane integrity, which is suppressed by osmotic support. Overexpression of several CWI pathway components suppress wsc1?Os zygotic death, including WSC2, WSC3, and BEM2, as well as the Rho-GAPS, BEM3 and RGD2. Microscopic observations and suppression by BEM2 and BEM3 suggest that wsc1?Os zygotes die during bud emergence. Downstream in the CWI pathway, overexpression of a hyperactive protein kinase C (Pkc1p-R398P) causes growth arrest, and blocks the pheromone response. With moderate levels of Pkc1p-R398P, cells form zygotes and the wsc1?Os defect is suppressed. This work highlights functional differences in the requirement for Wsc1p in diploids Versus haploids and between Mid2p and Wsc1p during mating" |
| Keywords: | cell wall integrity conjugation lysis ploidy protein kinase C yeast; |
| Notes: | "PubMed-not-MEDLINEHall, Allison E Lisci, Miriam Rose, Mark D eng R01 GM037739/GM/NIGMS NIH HHS/ R35GM126998/NH/NIH HHS/ R35 GM126998/GM/NIGMS NIH HHS/ GM007388/NH/NIH HHS/ R01GM037739/National Institute of Health/ Switzerland 2021/12/25 J Fungi (Basel). 2021 Dec 8; 7(12):1049. doi: 10.3390/jof7121049" |
