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Microbiol Res

Title:		"Avocado rhizobacteria emit volatile organic compounds with antifungal activity against Fusarium solani, Fusarium sp. associated with Kuroshio shot hole borer, and Colletotrichum gloeosporioides"
Author(s):		Guevara-Avendano E; Bejarano-Bolivar AA; Kiel-Martinez AL; Ramirez-Vazquez M; Mendez-Bravo A; von Wobeser EA; Sanchez-Rangel D; Guerrero-Analco JA; Eskalen A; Reverchon F;
Address:		"Red de Estudios Moleculares Avanzados, Instituto de Ecologia, A.C. Carretera antigua a Coatepec 351, Col. El Haya, 91070, Xalapa, Veracruz, Mexico; Instituto de Agroindustrias, Universidad Tecnologica de la Mixteca, Carretera a Acatlima Km. 2.5, Acatlima, 69000, Huajuapan de Leon, Oaxaca, Mexico. Electronic address: edkarguevaraa@gmail.com. Red de Estudios Moleculares Avanzados, Instituto de Ecologia, A.C. Carretera antigua a Coatepec 351, Col. El Haya, 91070, Xalapa, Veracruz, Mexico. Electronic address: alixadriana@gmail.com. Red de Estudios Moleculares Avanzados, Instituto de Ecologia, A.C. Carretera antigua a Coatepec 351, Col. El Haya, 91070, Xalapa, Veracruz, Mexico. Electronic address: ana.kiel@inecol.mx. Red de Estudios Moleculares Avanzados, Instituto de Ecologia, A.C. Carretera antigua a Coatepec 351, Col. El Haya, 91070, Xalapa, Veracruz, Mexico. Electronic address: monica.ramirez@inecol.mx. CONACYT - Escuela Nacional de Estudios Superiores, Laboratorio Nacional de Analisis y Sintesis Ecologica, Universidad Nacional Autonoma de Mexico, Antigua Carretera a Patzcuaro 8701, Col. Ex-Hacienda de San Jose de La Huerta, 58190, Morelia, Michoacan, Mexico. Electronic address: amendezbravo@enesmorelia.unam.mx. CONACYT - Centro de Investigacion y Desarrollo en Agrobiotecnologia Alimentaria (Consortium between Centro de Investigacion y Desarrollo, A.C. and Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco), Blvd. Sta. Catarina s/n, Col. Santiago Tlapacoya, 42110, San Agustin Tlaxiaca, Hidalgo, Mexico. Electronic address: eneas.aguirre@ciad.mx. Red de Estudios Moleculares Avanzados, Instituto de Ecologia, A.C. Carretera antigua a Coatepec 351, Col. El Haya, 91070, Xalapa, Veracruz, Mexico; CONACYT - Instituto de Ecologia, A.C. Carretera antigua a Coatepec 351, Col. El Haya, 91070, Xalapa, Veracruz, Mexico. Electronic address: diana.sanchez@inecol.mx. Red de Estudios Moleculares Avanzados, Instituto de Ecologia, A.C. Carretera antigua a Coatepec 351, Col. El Haya, 91070, Xalapa, Veracruz, Mexico. Electronic address: joseantonio.guerrero@inecol.mx. Department of Plant Pathology, Universidad de California - Davis, One Shields Avenue, Davis, CA, 95616-8751, United States. Electronic address: aeskalen@ucdavis.edu. Red de Estudios Moleculares Avanzados, Instituto de Ecologia, A.C. Carretera antigua a Coatepec 351, Col. El Haya, 91070, Xalapa, Veracruz, Mexico. Electronic address: frederique.reverchon@inecol.mx"
Journal Title:		Microbiol Res
Year:		2019
Volume:		20181124
Issue:
Page Number:		74 - 83
DOI:		10.1016/j.micres.2018.11.009
ISSN/ISBN:		1618-0623 (Electronic) 0944-5013 (Linking)
Abstract:		"Recent studies showed that bacterial volatile organic compounds (VOCs) play an important role in the suppression of phytopathogens. The ability of VOCs produced by avocado (Persea americana Mill.) rhizobacteria to suppress the growth of common avocado pathogens was therefore investigated. We evaluated the antifungal activity of VOCs emitted by avocado rhizobacteria in a first screening against Fusarium solani, and in subsequent antagonism assays against Fusarium sp. associated with Kuroshio shot hole borer, Colletotrichum gloeosporioides and Phytophthora cinnamomi, responsible for Fusarium dieback, anthracnosis and Phytophthora root rot in avocado, respectively. We also analyzed the composition of the bacterial volatile profiles by solid phase microextraction (SPME) gas chromatography coupled to mass spectrometry (GC-MS). Seven isolates, belonging to the bacterial genera Bacillus and Pseudomonas, reduced the mycelial growth of F. solani with inhibition percentages higher than 20%. Isolate HA, related to Bacillus amyloliquefaciens, significantly reduced the mycelial growth of Fusarium sp. and C. gloeosporioides and the mycelium density of P. cinnamomi. Isolates SO and SJJ, also members of the genus Bacillus, reduced Fusarium sp. mycelial growth and induced morphological alterations of fungal hyphae whilst isolate HB, close to B. mycoides, inhibited C. gloeosporioides. The analysis of the volatile profiles revealed the presence of ketones, pyrazines and sulfur-containing compounds, previously reported with antifungal activity. Altogether, our results support the potential of avocado rhizobacteria to act as biocontrol agents of avocado fungal pathogens and emphasize the importance of Bacillus spp. for the control of emerging avocado diseases such as Fusarium dieback"
Keywords:		Animals Antifungal Agents/metabolism/*pharmacology Bacillus/*metabolism Biological Control Agents/metabolism/*pharmacology Coleoptera/microbiology Colletotrichum/*drug effects Fusarium/*drug effects Persea/*microbiology Phytophthora/*drug effects Pseudomo;
Notes:		"MedlineGuevara-Avendano, Edgar Bejarano-Bolivar, Alix Adriana Kiel-Martinez, Ana-Luisa Ramirez-Vazquez, Monica Mendez-Bravo, Alfonso von Wobeser, Eneas Aguirre Sanchez-Rangel, Diana Guerrero-Analco, Jose A Eskalen, Akif Reverchon, Frederique eng Germany 2019/01/16 Microbiol Res. 2019 Feb; 219:74-83. doi: 10.1016/j.micres.2018.11.009. Epub 2018 Nov 24"