| Title: | "Identification of Gnr1p, a negative regulator of G alpha signalling in Schizosaccharomyces pombe, and its complementation by human G beta subunits" |
| Author(s): | Goddard A; Ladds G; Forfar R; Davey J; |
| Address: | "Department of Biological Sciences, University of Warwick, Coventry, UK. A.D.Goddard@warwick.ac.uk" |
| DOI: | 10.1016/j.fgb.2006.06.005 |
| ISSN/ISBN: | 1087-1845 (Print) 1087-1845 (Linking) |
| Abstract: | "G protein-coupled receptors (GPCRs) are involved in the response of eukaryotic cells to a wide variety of stimuli, traditionally mediating their effects through heterotrimeric G proteins comprised of G alpha, G beta and G gamma subunits. The fission yeast Schizosaccharomyces pombe is an established tool for GPCR research, possessing two G alpha-dependent signalling cascades. A complete G alpha beta gamma complex has been characterised for the glucose-sensing pathway, but only the G alpha subunit, Gpa1p, has been identified in the pheromone-response pathway. Here, we report the use of the yeast two-hybrid system to identify a novel protein, Gnr1p, which interacts with Gpa1p. Gnr1p is predicted to contain seven WD repeats and to adopt a structure similar to typical G beta subunits. Disruption and overexpression studies reveal that Gnr1p negatively regulates the pheromone-response pathway but is not required for signalling. Human G beta subunits complement the loss of Gnr1p, functioning as negative regulators of G alpha signalling in fission yeast" |
| Keywords: | "Amino Acid Sequence Base Sequence GTP-Binding Protein beta Subunits/*genetics/metabolism Gene Expression Regulation, Fungal/*genetics Genetic Complementation Test Humans Molecular Sequence Data Nucleic Acid Amplification Techniques Oligonucleotides Pherom;" |
| Notes: | "MedlineGoddard, Alan Ladds, Graham Forfar, Rachel Davey, John eng Comparative Study Research Support, Non-U.S. Gov't 2006/08/04 Fungal Genet Biol. 2006 Dec; 43(12):840-51. doi: 10.1016/j.fgb.2006.06.005. Epub 2006 Aug 1" |
