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New Phytol

Title:		"A multicomponent, elicitor-inducible cystatin complex in tomato, Solanum lycopersicum"
Author(s):		Girard C; Rivard D; Kiggundu A; Kunert K; Gleddie SC; Cloutier C; Michaud D;
Address:		"Departement de phytologie, CRH/INAF, Universite Laval, Quebec, Canada G1K 7P4. Forestry and Agricultural Biotechnology Institute, Botany Department, University of Pretoria, Pretoria 0002, South Africa. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada K1A 0C6. Departement de biologie, Centre de recherche en horticulture, Universite Laval, Quebec QC, Canada G1K 7P4"
Journal Title:		New Phytol
Year:		2007
Volume:		173
Issue:		4
Page Number:		841 - 851
DOI:		10.1111/j.1469-8137.2007.01968.x
ISSN/ISBN:		0028-646X (Print) 0028-646X (Linking)
Abstract:		"We assessed the ability of the fungal elicitor arachidonic acid to induce cystatin genes in tomato (Solanum lycopersicum), using a cDNA expression library from arachidonate-treated leaves. The cDNAs of two novel cystatins were isolated, coding for an approx. 11-kDa protein, SlCYS10; and for a 23.6-kDa protein, SlCYS9, bearing an N-terminal signal peptide and a long, 11.5-kDa extension at the C terminus. Both genes were induced by arachidonate but not by methyl jasmonate, an inducer of the 88-kDa eight-unit cystatin, multicystatin, accumulated in the cytosol of leaf cells upon herbivory. A truncated form of SlCYS9, tSlCYS9, was produced by deletion of the C-terminal extension to assess the influence of this structural element on the cystatin moiety. As shown by kinetic and stability assays with recombinant variants expressed in Escherichia coli, deleting the extension influenced both the overall stability and inhibitory potency of SlCYS9 against cysteine proteases of herbivorous organisms. These findings provide evidence for a multicomponent elicitor-inducible cystatin complex in tomato, including at least 10 cystatin units produced via two metabolic routes"
Keywords:		"Acetates/*pharmacology Amino Acid Sequence Arachidonic Acids/*pharmacology Base Sequence Cyclopentanes/*pharmacology Cystatins/chemistry/*genetics/pharmacology Cysteine Proteinase Inhibitors/genetics/pharmacology DNA, Complementary DNA, Plant Gene Deletio;"
Notes:		"MedlineGirard, Cecile Rivard, Daniel Kiggundu, Andrew Kunert, Karl Gleddie, Stephen C Cloutier, Conrad Michaud, Dominique eng Research Support, Non-U.S. Gov't England 2007/02/09 New Phytol. 2007; 173(4):841-851. doi: 10.1111/j.1469-8137.2007.01968.x"