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J Bacteriol

Title:		Genetic structure of the Enterococcus faecalis plasmid pAD1-encoded cytolytic toxin system and its relationship to lantibiotic determinants
Author(s):		Gilmore MS; Segarra RA; Booth MC; Bogie CP; Hall LR; Clewell DB;
Address:		"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190"
Journal Title:		J Bacteriol
Year:		1994
Volume:		176
Issue:		23
Page Number:		7335 - 7344
DOI:		10.1128/jb.176.23.7335-7344.1994
ISSN/ISBN:		0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking)
Abstract:		"Pheromone-responsive conjugative plasmids are unique to the species Enterococcus faecalis. Many pheromone-responsive plasmids, including those frequently isolated from sites of infection, express a novel cytolysin that possesses both hemolytic and bacteriocin activities. Further, this cytolysin has been shown to be a toxin in several disease models. In the present study, nucleotide sequence determination, mutagenesis, and complementation analysis were used to determine the organization of the E. faecalis plasmid pAD1 cytolysin determinant. Four open reading frames are required for expression of the cytolysin precursor (cylLL, cylLS, cylM, and cylB). The inferred products of two of these open reading frames, CyILL and CyILS, constitute the cytolysin precursor and bear structural resemblance to posttranslationally modified bacteriocins termed lantibiotics. Similarities between the organization of the E. faecalis cytolysin determinant and expression units for lantibiotics exist, indicating that the E. faecalis cytolysin represents a new branch of this class and is the first known to possess toxin activity"
Keywords:		Adenosine Triphosphate/metabolism Amino Acid Sequence Bacterial Proteins/genetics Bacterial Toxins/*genetics Bacteriocins/*genetics Base Sequence Cytotoxins/biosynthesis/*genetics Enterococcus faecalis/*genetics Gene Expression Genetic Complementation Tes;
Notes:		"MedlineGilmore, M S Segarra, R A Booth, M C Bogie, C P Hall, L R Clewell, D B eng AI10318/AI/NIAID NIH HHS/ EY08289/EY/NEI NIH HHS/ GM33956/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1994/12/01 J Bacteriol. 1994 Dec; 176(23):7335-44. doi: 10.1128/jb.176.23.7335-7344.1994"