| Title: | Age-related trends in gene expression in the chemosensory-nasal mucosae of senescence-accelerated mice |
| Author(s): | Getchell TV; Peng X; Stromberg AJ; Chen KC; Paul Green C; Subhedar NK; Shah DS; Mattson MP; Getchell ML; |
| Address: | "Department of Physiology, 309 Sanders-Brown Center on Aging, University of Kentucky, 800 South Limestone Street, Lexington, KY 40536-0230, USA. tgetche@uky.edu" |
| DOI: | 10.1016/s1568-1637(02)00066-1 |
| ISSN/ISBN: | 1568-1637 (Print) 1568-1637 (Linking) |
| Abstract: | "We have utilized high-density GeneChip oligonucleotide arrays to investigate the use of the senescence-accelerated mouse (SAM) as a biogerontological resource to identify patterns of gene expression in the chemosensory-nasal mucosa. Gene profiling in chronologically young and old mice of the senescence-resistant (SAMR) and senescence-prone (SAMP) strains revealed 133 known genes that were modulated by a three-fold or greater change either in one strain or the other or in both strains during aging. We also identified known genes in our study which based on their encoded proteins were identified as aging-related genes in the aging neocortex and cerebellum of mice as reported by Lee et al. (2000) [Nat. Genet. 25 (2000) 294]. Changes in gene profiles for chemosensory-related genes including olfactory and vomeronasal receptors, sensory transduction-associated proteins, and odor and pheromone transport molecules in the young SAMR and SAMP were compared with age-matched C57BL/6J mice. An analysis of known gene expression profiles suggests that changes in the expression of immune factor genes and genes associated with cell cycle progression and cell death were particularly prominent in the old SAM strains. A preliminary cellular validation study supported the dysregulation of cell cycle-related genes in the old SAM strains. The results of our initial study indicated that the use of the SAM models of aging could provide substantive information leading to a more fundamental understanding of the aging process in the chemosensory-nasal mucosa at the genomic, molecular, and cellular levels" |
| Keywords: | "Aging/*genetics Animals Cerebellum/physiology Chemoreceptor Cells/*metabolism *Gene Expression Gene Expression Profiling Gene Expression Regulation Mice Mice, Inbred C57BL Mice, Inbred Strains Nasal Cavity/metabolism Nasal Mucosa/*metabolism Nasal Septum/;" |
| Notes: | "MedlineGetchell, Thomas V Peng, Xuejun Stromberg, Arnold J Chen, Kuey-Chu Paul Green, C Subhedar, Nishikant K Shah, Dharmen S Mattson, Mark P Getchell, Marilyn L eng 1P20RR16481-01/RR/NCRR NIH HHS/ AG-016824-22/AG/NIA NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. England 2003/02/28 Ageing Res Rev. 2003 Apr; 2(2):211-43. doi: 10.1016/s1568-1637(02)00066-1" |
