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J Biol Chem

Title:		Comparative topology studies in Saccharomyces cerevisiae and in Escherichia coli. The N-terminal half of the yeast ABC protein Ste6
Author(s):		Geller D; Taglicht D; Edgar R; Tam A; Pines O; Michaelis S; Bibi E;
Address:		"Department of Biochemistry, Weizmann Institute of Science, Rehovot 76100, Israel"
Journal Title:		J Biol Chem
Year:		1996
Volume:		271
Issue:		23
Page Number:		13746 - 13753
DOI:		10.1074/jbc.271.23.13746
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"Gene fusions have provided a strategy for determining the topology of polytopic membrane proteins in Escherichia coli. To evaluate whether this highly effective approach is applicable to heterologously expressed eukaryotic integral membrane proteins, we have carried out a comparative topological study of the eukaryotic membrane protein Ste6 both in bacteria and in yeast. Ste6, is an ATP binding cassette (ABC) protein, essential for export of the a-factor mating pheromone in Saccharomyces cerevisiae. The topogenic reporters, invertase in S. cerevisiae and alkaline phosphatase in E. coli, were fused to Ste6 at identical sites and the fusions were expressed in yeast and bacteria, respectively. The results obtained in both systems are similar, although more definitive in E. coli, and support the predicted six-transmembrane spans organization of the N-terminal half of Ste6. Thus, the topological determinants for membrane insertion of polytopic proteins in prokaryotic and in eukaryotic systems appear to be highly similar. In this study we also demonstrate that Ste6 does not contain a cleaved signal sequence"
Keywords:		"ATP-Binding Cassette Transporters/*chemistry/genetics Alkaline Phosphatase/genetics Cloning, Molecular Escherichia coli/*chemistry/genetics Fungal Proteins/*chemistry/genetics Genes, Bacterial Genes, Fungal Genes, Reporter *Glycoproteins Glycoside Hydrola;"
Notes:		"MedlineGeller, D Taglicht, D Edgar, R Tam, A Pines, O Michaelis, S Bibi, E eng GM51508/GM/NIGMS NIH HHS/ P50 DK48977/DK/NIDDK NIH HHS/ Comparative Study Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1996/06/07 J Biol Chem. 1996 Jun 7; 271(23):13746-53. doi: 10.1074/jbc.271.23.13746"