| Title: | Effects of lipopolysaccharide and juvenile hormone III treatments on cell growth and gene expression in the Ceratitis capitata (Diptera: Tephritidae) CCE/CC128 cell line |
| Author(s): | Garcia-Reina A; Rossi E; Galian J; |
| Address: | "Department of Zoology and Physical Anthropology, Faculty of Veterinary, Campus Mare Nostrum, University of Murcia, Murcia, Spain. DAFE, University of Pisa, Pisa, Italy" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 1520-6327 (Electronic) 0739-4462 (Linking) |
| Abstract: | "The Mediterranean fruit fly Ceratitis capitata is one of the most important insect pest species in the world. It has a high colonization capacity and population variety, giving it considerable genetic diversity. Strategies for its control have included the sterile insect technique and insect growth regulators. Many studies have analyzed the medfly transcriptome, and along with the medfly genome sequence, the sequences of multiple genes related to sex determination, mating, development, pheromone detection, immunity, or stress have been identified. In this study, the medfly CCE/CC128 cell line was used to assess cell growth variation and changes in the expression of genes covering different functions, after lipopolysaccharide (LPS) and juvenile hormone III (JHIII) treatments. No significant effects on cell growth and gene expression were observed in the cells treated with LPS. In the cells treated with JHIII, the results showed significant effects on cell growth, and an overexpression was found of the Shade gene, one of the Halloween gene members of the cytochrome p450 family, which is involved in development and the synthesis of 20-hydroxyecdysone. This study shows preliminary results on the insect cell line in combination with whole-genome sequencing, which can facilitate studies regarding growth, toxicity, immunity, and transcriptome regulations as a response to different compounds and environmental alterations" |
| Keywords: | Animals Cell Line Cell Proliferation/drug effects Ceratitis capitata/cytology/*drug effects/genetics/metabolism Gene Expression/drug effects Lipopolysaccharides/*pharmacology Sesquiterpenes/*pharmacology cell challenging development genome insect cell lin; |
| Notes: | "MedlineGarcia-Reina, Andres Rossi, Elisabetta Galian, Jose eng 2019/09/13 Arch Insect Biochem Physiol. 2019 Dec; 102(4):e21617. doi: 10.1002/arch.21617. Epub 2019 Sep 12" |
