| Title: | Genomic footprinting of the promoter regions of STE2 and STE3 genes in the yeast Saccharomyces cerevisiae |
| Author(s): | Ganter B; Tan S; Richmond TJ; |
| Address: | "Institut fur Molekularbiologie und Biophysik, ETH-Honggerberg, Zurich, Switzerland" |
| ISSN/ISBN: | 0022-2836 (Print) 0022-2836 (Linking) |
| Abstract: | "Dimethyl sulfate, DNase I and micrococcal nuclease DNA cleavage were combined with the ligation-mediated polymerase chain reaction to obtain high resolution maps of the promoter regions for two cell-type-specific genes: the a-specific STE2 gene and the alpha-specific STE3 gene. We find that MCM1 binds in vivo in a-cells to a 16 bp P-box sequence located in the STE2 UAS. In alpha-cells, the footprint pattern is extended relative to a-cells, consistent with the additional binding of MAT alpha 2 to the sequences flanking each end of the P-box. A nucleosome was found adjacent to the P-box of the transcriptionally repressed a-specific STE2 UAS in alpha-cells, positioned so that the nucleosome overlaps the TATA-box. In contrast, such well-positioned nucleosomes were not found for the transcriptionally active STE2 UAS in a-cells, where instead the TATA box appears to be bound to the general transcription factor TFIID. These observations support the hypothesis that MAT alpha 2 repression of a-specific genes is mediated by nucleosomes, perhaps by exclusion of TFIID from the TATA-box" |
| Keywords: | "Base Sequence DNA Primers/chemistry Fungal Proteins/genetics *Gene Expression Regulation, Fungal Genes, Fungal Molecular Sequence Data Nucleosomes/ultrastructure *Promoter Regions, Genetic RNA, Messenger/genetics Receptors, Cell Surface/*genetics *Recepto;" |
| Notes: | "MedlineGanter, B Tan, S Richmond, T J eng Netherlands 1993/12/20 J Mol Biol. 1993 Dec 20; 234(4):975-87. doi: 10.1006/jmbi.1993.1652" |
