| Title: | Behavioural and electrophysiological responses of Triatoma dimidiata nymphs to conspecific faecal volatiles |
| Author(s): | Galvez-Marroquin Z; Cruz-Lopez L; Malo EA; Ramsey JM; Rojas JC; |
| Address: | "Grupo de Ecologia de Artropodos y Manejo de Plagas, El Colegio de la Frontera Sur (ECOSUR), Tapachula, Chiapas, Mexico. Centro Regional de Investigacion en Salud Publica del Instituto Nacional de Salud Publica (CRISP-INSP), Tapachula, Chiapas, Mexico" |
| ISSN/ISBN: | 1365-2915 (Electronic) 0269-283X (Linking) |
| Abstract: | "The behavioural and electrophysiological (electroantennography) responses of the first two instars of Triatoma dimidiata (Hemiptera: Reduviidae) Latreille to fresh and dry faecal headspace volatile extracts from fifth instar conspecific nymphs and synthetic compounds were analysed in this study. Recently emerged nymphs (3-5 days) aggregated around filter paper impregnated with dry faeces and around filter paper impregnated with extracts from both fresh and dry faeces. Older first instars (10-15 days) and second instars aggregated around filter paper impregnated with fresh and dry faeces, and their respective headspace extracts. Dry faecal volatile extracts elicited the strongest antennal responses, followed by fresh faecal extracts. Gas chromatography-mass spectrometry analysis of dried faecal headspace volatiles demonstrated the presence of 12 compounds: 2-ethyl-1-hexanol, 1,2,4-trimethylbenzene, n-octadecane, n-nonadecane, n-eicosane, n-heneicosane, n-tricosane, n-pentaeicosane, n-hexaeicosane, n-octaeicosane, nonanal, and 4-methyl quinazoline. In fresh faecal headspace extracts, only nonanal was clearly detected, although there were other trace compounds, including several unidentified sesquiterpenes. Four of the 11 compounds tested individually elicited aggregation behaviour at concentrations of 100 ng/microL and 1 microg/microL. A blend containing these four components also mediated the aggregation of nymphs. These volatiles may be valuable for developing monitoring methods and designing sensitive strategies to detect and measure T. dimidiata infestation" |
| Keywords: | Animals Arthropod Antennae/*physiology *Chemotaxis Electrophysiological Phenomena Feces/*chemistry Nymph/growth & development/physiology Social Behavior Triatoma/growth & development/*physiology Volatile Organic Compounds/*analysis Aggregation Chagas dise; |
| Notes: | "MedlineGalvez-Marroquin, Z Cruz-Lopez, L Malo, E A Ramsey, J M Rojas, J C eng Research Support, Non-U.S. Gov't England 2017/09/12 Med Vet Entomol. 2018 Mar; 32(1):102-110. doi: 10.1111/mve.12271. Epub 2017 Sep 11" |
