| Title: | A locus coding for putative non-ribosomal peptide/polyketide synthase functions is mutated in a swarming-defective Proteus mirabilis strain |
| Address: | "University of Cambridge, Department of Pathology, UK" |
| ISSN/ISBN: | 0026-8925 (Print) 0026-8925 (Linking) |
| Abstract: | "We describe a large bacterial locus that, unusually, encodes components typically required for both the non-ribosomal synthesis of peptides and also polyketide/fatty acid synthase function. Two tandem ABC transporter genes in this putative nrp (non-ribosomal peptide/polyketide) operon suggest that the principal product may be secreted. Immediately distal to the nrp operon is a gene, irpP, encoding a small peptide similar to the Bacillus ComX pheromone that in its mature, extracellular form increases expression of unlinked non-ribosomal peptide synthesis genes. Transcription of both the nrp operon and irpP was up-regulated in iron-limiting culture conditions, consistent with the presence of a putative Fur repressor-binding site 5' of irpP. The locus was isolated from Proteus mirabilis as the site of a TnphoA insertion causing impaired swarm cell differentiation and an aberrant swarming pattern. The mutation was in one of the transporter genes, but a comparable swarming defect resulted from interposon disruption of the putative nrp synthetase gene" |
| Keywords: | "ATP-Binding Cassette Transporters/genetics Amino Acid Sequence Bacterial Outer Membrane Proteins/chemistry/genetics *Bacterial Proteins Base Sequence Gene Expression Regulation, Bacterial Iron/metabolism Iron-Binding Proteins Molecular Sequence Data Movem;" |
| Notes: | "MedlineGaisser, S Hughes, C eng Wellcome Trust/United Kingdom Research Support, Non-U.S. Gov't Germany 1997/01/27 Mol Gen Genet. 1997 Jan 27; 253(4):415-27. doi: 10.1007/s004380050339" |
