| Title: | Development of a novel comprehensive analytical method for volatile compounds using supercritical fluid chromatography/mass spectrometry with a highly cross-linked styrene divinylbenzene polymer-based column |
| Author(s): | Fujito Y; Hayakawa Y; Bamba T; |
| Address: | "Division of Analytical and Measuring Instruments, Shimadzu Corporation, 1 Kuwabaracho Nishinokyo Nakagyo-ku, Kyoto, 604-8511, Japan; Division of Metabolomics, Research Center for Transomics Medicine, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi Higashi-ku, Fukuoka, 812-8582, Japan. Division of Analytical and Measuring Instruments, Shimadzu Corporation, 1 Kuwabaracho Nishinokyo Nakagyo-ku, Kyoto, 604-8511, Japan. Division of Metabolomics, Research Center for Transomics Medicine, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi Higashi-ku, Fukuoka, 812-8582, Japan. Electronic address: bamba@bioreg.kyushu-u.ac.jp" |
| DOI: | 10.1016/j.chroma.2020.461363 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "Analytical techniques to determine volatile compounds such as flavor, aroma, and fragrances are in high demand due to their wide range of applications in industry, the chemical properties of them are very diverse. Supercritical fluid chromatography (SFC) is capable of high speed, high peak capacity separation and has a high separation coverage. It is also an advantageous for preparative purifications due to its unique mobile phase conditions. However, there is no column commercially available for SFC that is suitable to comprehensively separate volatile compounds. SFC is limited to the use of silica-based columns due to weak retentions and polymer-based column issues such as pressure, swelling and shrinkage tolerances. This study demonstrated comprehensive analytical method for volatile in SFC using a highly cross-linked styrene divinylbenzene (SDVB) polymer-based column, newly developed for SFC. In this study, 23 typical volatile compounds with a wide variety of chemical properties were selected as model compounds. The newly developed SDVB column showed, compared to conventional silica-based columns (k > 0.3), an excellent overall and substantial improved retentions (k > 1.6) under SFC mobile phase conditions. It was also able to retain esters (hydroxy acetate, pentyl butylate, methyl salicylate) and non-polar terpenes (limonene, pinene) that did not show sufficient retention in any other commercially available silica-based columns. Aldehydes reacting on NH(2) column due to Schiff base formation were also successfully eluted. It was confirmed that SDVB column provided comprehensive separation and wide coverage for volatile compounds" |
| Keywords: | "Chromatography, Supercritical Fluid/*methods Mass Spectrometry Polystyrenes/*chemistry Silicon Dioxide Terpenes/isolation & purification Vinyl Compounds/*chemistry Volatile Organic Compounds/analysis/isolation & purification Styrene divinylbenzene polymer;" |
| Notes: | "MedlineFujito, Yuka Hayakawa, Yoshihiro Bamba, Takeshi eng Netherlands 2020/08/17 J Chromatogr A. 2020 Aug 30; 1626:461363. doi: 10.1016/j.chroma.2020.461363. Epub 2020 Jun 22" |
