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Curr Genet

Title:		Molecular cloning of the DAC2/FUS3 gene essential for pheromone-induced G1-arrest of the cell cycle in Saccharomyces cerevisiae
Author(s):		Fujimura H;
Address:		"Laboratory for Molecular Biology, Hoechst Japan Ltd., Kawagoe, Japan"
Journal Title:		Curr Genet
Year:		1990
Volume:		18
Issue:		5
Page Number:		395 - 400
DOI:		10.1007/BF00309907
ISSN/ISBN:		0172-8083 (Print) 0172-8083 (Linking)
Abstract:		"Mating pheromones, known as a and alpha-factors, arrest the division of cells of opposite mating types, alpha and a respectively, in Saccharomyces cerevisiae. I have cloned the DAC2 gene, which is required for both pheromone-induced division-arrest and cell-fusion during conjugation. The constructed dac2::LEU2 null mutation leads to defects in both pheromone-induced division-arrest and cell-fusion during conjugation; it also suppresses the growth defect caused by the gpa1 mutation (a mutation in the alpha subunit of the S. cerevisiae G protein). These results indicate that DAC2 may be the same gene as FUS3, which was recently isolated by Elion et al. (1990) as a gene essential for cell-fusion during conjugation. The dac2::LEU2 null mutant also showed morphological alterations in response to mating pheromones. I show here that the DAC2 product plays an essential role in both the division-arrest signalling pathway of the yeast pheromone response and in cell-fusion during conjugation"
Keywords:		"Cloning, Molecular G1 Phase Genes, Fungal Mating Factor Mutation Peptides/*pharmacology Phenotype Pheromones/*pharmacology Restriction Mapping Saccharomyces cerevisiae/cytology/*genetics Signal Transduction Suppression, Genetic;"
Notes:		"MedlineFujimura, H eng 1990/12/01 Curr Genet. 1990 Dec; 18(5):395-400. doi: 10.1007/BF00309907"