| Title: | Multiple Delta11-desaturase genes selectively used for sex pheromone biosynthesis are conserved in Ostrinia moth genomes |
| Author(s): | Fujii T; Yasukochi Y; Rong Y; Matsuo T; Ishikawa Y; |
| Address: | "Laboratory of Applied Entomology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: takeshi.f0405@gmail.com. Insect Genome Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan. Laboratory of Applied Entomology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan" |
| DOI: | 10.1016/j.ibmb.2015.04.007 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "Regulation of the expression of fatty acyl-CoA desaturases, which introduce a double bond into the fatty acid moiety of the substrate, is crucial for the production of species-specific sex pheromones in moths. In Ostrinia moths, two distinct Delta11-desaturases and a Delta14-desaturase are known to be selectively used in the biosynthesis of sex pheromones. Of the two Delta11-desaturases, one identified from Ostrinia nubilalis and Ostrinia scapulalis, Z/EDelta11, forms the Z and E isomers of a double bond at position 11, whereas the other identified from Ostrinia latipennis, LATPG1(=EDelta11), exclusively forms an E double bond at position 11. Since the retroposon(ezi)-fused, non-functional Delta11-desaturase gene, ezi-Delta11alpha, in the genomes of O. nubilalis and O. furnacalis was previously suggested to be an orthologue of latpg1, we here explored Z/EDelta11 orthologues in the genome of O. latipennis. We newly identified two Delta11-desaturase genes, latpg2 and latpg3, which were orthologous to ezi-Delta11beta and Z/EDelta11, respectively. We found that an ezi-like element was integrated in intron 1 of latpg1, and confirmed that only latpg1 was expressed in the pheromone gland of O. latipennis. Thus, at least three Delta11-desaturase genes are present in the genome of O. latipennis, and latpg1 is selectively transcribed in the pheromone gland of this moth. The non-functionality of ezi-inserted desaturase genes in O. nubilalis and O. furnacalis may not be a direct consequence of the insertion of an ezi- or ezi-like element into the gene" |
| Keywords: | "Animals Base Sequence Fatty Acid Desaturases/genetics/*metabolism Female Genome, Insect Insect Proteins/genetics/*metabolism Molecular Sequence Data Moths/enzymology/*genetics Phylogeny Sex Attractants/*biosynthesis/genetics Species Specificity Genome str;" |
| Notes: | "MedlineFujii, Takeshi Yasukochi, Yuji Rong, Yu Matsuo, Takashi Ishikawa, Yukio eng Research Support, Non-U.S. Gov't England 2015/05/06 Insect Biochem Mol Biol. 2015 Jun; 61:62-8. doi: 10.1016/j.ibmb.2015.04.007. Epub 2015 May 2" |
