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Appl Biochem Biotechnol

Title:		Characterisation of a Recombinant Patchoulol Synthase Variant for Biocatalytic Production of Terpenes
Author(s):		Frister T; Hartwig S; Alemdar S; Schnatz K; Thons L; Scheper T; Beutel S;
Address:
Journal Title:		Appl Biochem Biotechnol
Year:		2015
Volume:		20150623
Issue:		8
Page Number:		2185 - 2201
DOI:		10.1007/s12010-015-1707-y
ISSN/ISBN:		1559-0291 (Electronic) 0273-2289 (Linking)
Abstract:		"The patchoulol synthase (PTS) is a multi-product sesquiterpene synthases which is the central enzyme for biosynthesis of patchouli essential oil in the patchouli plant. Sesquiterpene synthases catalyse the formation of various complex carbon backbones difficult to approach by organic synthesis. Here, we report the characterisation of a recombinant patchoulol synthase complementary DNA (cDNA) variant (PTS var. 1), exhibiting significant amino acid exchanges compared to the native PTS. The product spectrum using the natural substrate E,E-farnesyl diphosphate (FDP) as well as terpenoid products resulting from conversions employing alternative substrates was analysed by GC-MS. In respect to a potential use as a biocatalyst, important enzymatic parameters such as the optimal reaction conditions, kinetic behaviour and the product selectivity were studied as well. Adjusting the reaction conditions, an increased patchoulol ratio in the recombinant essential oil was achieved. Nevertheless, the ratio remained lower than in plant-derived patchouli oil. As alternative substrates, several prenyl diposphates were accepted and converted in numerous compounds by the PTS var. 1, revealing its great biocatalytic potential"
Keywords:		"*Biocatalysis Gas Chromatography-Mass Spectrometry Hydrogen-Ion Concentration Isomerases/*metabolism Kinetics Lamiaceae/enzymology Mutation/*genetics Oils, Volatile/metabolism Recombinant Proteins/*metabolism Substrate Specificity Temperature Terpenes/che;"
Notes:		"MedlineFrister, Thore Hartwig, Steffen Alemdar, Semra Schnatz, Katharina Thons, Laura Scheper, Thomas Beutel, Sascha eng Research Support, Non-U.S. Gov't 2015/06/24 Appl Biochem Biotechnol. 2015 Aug; 176(8):2185-201. doi: 10.1007/s12010-015-1707-y. Epub 2015 Jun 23"