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Mol Biol Cell

Title:		Characterization of the yeast amphiphysins Rvs161p and Rvs167p reveals roles for the Rvs heterodimer in vivo
Author(s):		Friesen H; Humphries C; Ho Y; Schub O; Colwill K; Andrews B;
Address:		"Department of Medical Genetics and Microbiology, Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada M5S 1A8"
Journal Title:		Mol Biol Cell
Year:		2006
Volume:		20060104
Issue:		3
Page Number:		1306 - 1321
DOI:		10.1091/mbc.e05-06-0476
ISSN/ISBN:		1059-1524 (Print) 1059-1524 (Linking)
Abstract:		"We have used comprehensive synthetic lethal screens and biochemical assays to examine the biological role of the yeast amphiphysin homologues Rvs161p and Rvs167p, two proteins that play a role in regulation of the actin cytoskeleton, endocytosis, and sporulation. We found that unlike some forms of amphiphysin, Rvs161p-Rvs167p acts as an obligate heterodimer during vegetative growth and neither Rvs161p nor Rvs167p forms a homodimer in vivo. RVS161 and RVS167 have an identical set of 49 synthetic lethal interactions, revealing functions for the Rvs proteins in cell polarity, cell wall synthesis, and vesicle trafficking as well as a shared role in mating. Consistent with these roles, we show that the Rvs167p-Rvs161p heterodimer, like its amphiphysin homologues, can bind to phospholipid membranes in vitro, suggesting a role in vesicle formation and/or fusion. Our genetic screens also reveal that the interaction between Abp1p and the Rvs167p Src homology 3 (SH3) domain may be important under certain conditions, providing the first genetic evidence for a role for the SH3 domain of Rvs167p. Our studies implicate heterodimerization of amphiphysin family proteins in various functions related to cell polarity, cell integrity, and vesicle trafficking during vegetative growth and the mating response"
Keywords:		"Actins/metabolism Animals Carrier Proteins/metabolism Cattle Cell Line Cell Membrane/metabolism Chitin/metabolism Cytoskeletal Proteins/*chemistry/*metabolism Dimerization Diploidy Gene Deletion Genes, Mating Type, Fungal Genetic Complementation Test Inse;"
Notes:		"MedlineFriesen, Helena Humphries, Christine Ho, Yuen Schub, Oliver Colwill, Karen Andrews, Brenda eng Research Support, Non-U.S. Gov't 2006/01/06 Mol Biol Cell. 2006 Mar; 17(3):1306-21. doi: 10.1091/mbc.e05-06-0476. Epub 2006 Jan 4"