| Title: | Molecular analysis of the pheromone and pheromone receptor genes of Ustilago hordei |
| Author(s): | Anderson CM; Willits DA; Kosted PJ; Ford EJ; Martinez-Espinoza AD; Sherwood JE; |
| Address: | "Department of Plant Sciences, Montana State University, Bozeman, MT, USA" |
| DOI: | 10.1016/s0378-1119(99)00428-x |
| ISSN/ISBN: | 0378-1119 (Print) 0378-1119 (Linking) |
| Abstract: | "Cell-cell signaling is an integral part of the sexual and disease cycles of the smut fungi, which must mate to be pathogenic. This study reports the cloning and characterization of the pheromone genes Uhmfa1 and Uhmfa2 from MAT-1 and MAT-2 mating types of U. hordei, respectively, and the pheromone receptor gene Uhpra2 from MAT-2 cells. Similar to other fungal pheromone genes, Uhmfa1 and Uhmfa2 encode precursor peptides. Uhpra2 encodes a protein with sequence similarity to the 7-transmembrane class of G-protein coupled receptors. Deletion of Uhmfa1 and Uhpra1, and their subsequent replacement, confirmed the role of these genes in initiation of the sexual cycle. Uhmfa1 and Uhmfa2 were differentially expressed in various cell types and when opposite mating-type cells were grown together. The predicted mature pheromones of each mating type were synthesized, and each specifically induced conjugation tube formation in cells of the opposite mating type" |
| Keywords: | "Amino Acid Sequence Base Sequence *Chemoreceptor Cells Cloning, Molecular DNA, Fungal/chemistry/genetics *Fungal Proteins Gene Deletion Gene Expression Regulation, Fungal Genes, Fungal/*genetics *Genes, Mating Type, Fungal Molecular Sequence Data Pheromon;" |
| Notes: | "MedlineAnderson, C M Willits, D A Kosted, P J Ford, E J Martinez-Espinoza, A D Sherwood, J E eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Netherlands 1999/11/24 Gene. 1999 Nov 15; 240(1):89-97. doi: 10.1016/s0378-1119(99)00428-x" |
