« Previous AbstractFunctional role of TRPC proteins in vivo: lessons from TRPC-deficient mouse models    Next Abstract"Genetic screening of Lactobacillus sakei and Lactobacillus curvatus strains for their peptidolytic system and amino acid metabolism, and comparison of their volatilomes in a model system" »

J Physiol

Title:		Functional role of TRPC proteins in native systems: implications from knockout and knock-down studies
Author(s):		Freichel M; Vennekens R; Olausson J; Stolz S; Philipp SE; Weissgerber P; Flockerzi V;
Address:		"Experimentelle und Klinische Pharmakologie und Toxikologie, Universitat des Saarlandes, D 66421 Homburg, Germany. marc.freichel@uniklinik-saarland.de"
Journal Title:		J Physiol
Year:		2005
Volume:		20050623
Issue:		Pt 1
Page Number:		59 - 66
DOI:		10.1113/jphysiol.2005.092999
ISSN/ISBN:		0022-3751 (Print) 1469-7793 (Electronic) 0022-3751 (Linking)
Abstract:		"Available data on transient receptor potential channel (TRPC) protein functions indicate that these proteins represent essential constituents of agonist-activated and phospholipase C-dependent cation entry pathways in primary cells which contribute to the elevation of cytosolic Ca2+. In addition, a striking number of biological functions have already been assigned to the various TRPC proteins, including mechanosensing activity (TRPC1), chemotropic axon guidance (TRPC1 and TRPC3), pheromone sensing and the regulation of sexual and social behaviour (TRPC2), endothelial-dependent regulation of vascular tone, endothelial permeability and neurotransmitter release (TRPC4), axonal growth (TRPC5), modulation of smooth muscle tone in blood vessels and lung and regulation of podocyte structure and function in the kidney (TRPC6). The lack of compounds which specifically block or activate TRPC proteins impairs the analysis of TRPC function in primary cells. We therefore concentrate in this contribution on (i) studies of TRPC-deficient mouse lines, (ii) data obtained by gene-silencing approaches using antisense oligonucleotides or RNA interference, (iii) expression experiments employing dominant negative TRPC constructs, and (iv) recent data correlating mutations of TRPC genes associated with human disease"
Keywords:		"Animals Calcium Channels/*genetics/*physiology Gene Silencing Humans Ion Channels/genetics/physiology Mice *Mice, Knockout TRPC Cation Channels;"
Notes:		"MedlineFreichel, Marc Vennekens, Rudi Olausson, Jenny Stolz, Susanne Philipp, Stephan E Weissgerber, Petra Flockerzi, Veit eng Consensus Development Conference Research Support, Non-U.S. Gov't Review England 2005/06/25 J Physiol. 2005 Aug 15; 567(Pt 1):59-66. doi: 10.1113/jphysiol.2005.092999. Epub 2005 Jun 23"