| Title: | "Behavioral response of the malaria mosquito, Anopheles gambiae, to human sweat inoculated with axilla bacteria and to volatiles composing human axillary odor" |
| Author(s): | Frei J; Krober T; Troccaz M; Starkenmann C; Guerin PM; |
| Address: | "Institute of Biology, University of Neuchatel, Rue Emile-Argand 11, 2000 Neuchatel, Switzerland and. Corporate Research and Development Division, Firmenich S.A., PO Box 239, 1211 Geneva 8, Switzerland. Institute of Biology, University of Neuchatel, Rue Emile-Argand 11, 2000 Neuchatel, Switzerland and patrick.guerin@unine.ch" |
| ISSN/ISBN: | 1464-3553 (Electronic) 0379-864X (Linking) |
| Abstract: | "The responses of Anopheles gambiae Giles sensu stricto (Diptera: Culicidae) to odors from male and female axillary sweat incubated with human axilla bacteria were recorded in a dual-choice olfactometer. Staphylococcus epidermidis was selected for its low odor-producing pattern, Corynebacterium jeikeium for its strong Nalpha-acylglutamine aminoacylase activity liberating carboxylic acids including (R)/(S)-3-hydroxy-3-methylhexanoic acid (HMHA) and Staphylococcus haemolyticus for its capacity to liberate sulfur-containing compounds including (R/S)-3-methyl-3-sulfanylhexan-1-ol (MSH). Anopheles gambiae behavioral responses were evaluated under (i) its responsiveness to take off and undertake sustained upwind flight and (ii) its discriminating capacity between the two olfactometer arms bearing a test odor in either one or both arms. Experiments were conducted in the presence of carbon dioxide pulses as a behavioral sensitizer. Anopheles gambiae clearly discriminated for the olfactometer arm conveying odor generated by incubating any of the three bacteria species with either male or female sweat. Whereas An. gambiae did not discriminate between male and female sterile sweat samples in the olfactometer, the mosquito consistently showed a preference for male sweat over female sweat incubated with the same bacterium, independent of the species used as inoculum. Sweat incubated with C. jeikeium rendered mosquitoes particularly responsive and this substrate elicited the strongest preference for male over female sweat. Tested on their own, neither HMHA nor MSH elicited a clear discriminating response but did affect mosquito responsiveness. These findings serve as a basis for further research on the odor-mediated anthropophilic host-seeking behavior of An. gambiae" |
| Keywords: | Animals Anopheles/*physiology Axilla/*microbiology Bacteria/*metabolism Female Humans Male Odorants/*analysis Pheromones/*physiology Sweat/*chemistry Anopheles gambiae (R)/(S)-3-hydroxy-3-methylhexanoic acid (R/S)-3-methyl-3-sulfanylhexan-1-ol axilla bact; |
| Notes: | "MedlineFrei, Jerome Krober, Thomas Troccaz, Myriam Starkenmann, Christian Guerin, Patrick M eng England 2016/10/30 Chem Senses. 2017 Feb; 42(2):121-131. doi: 10.1093/chemse/bjw106. Epub 2016 Oct 27" |
