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Mol Biol Cell

Title:		Multiple sex pheromones and receptors of a mushroom-producing fungus elicit mating in yeast
Author(s):		Fowler TJ; DeSimone SM; Mitton MF; Kurjan J; Raper CA;
Address:		"Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont 05405, USA"
Journal Title:		Mol Biol Cell
Year:		1999
Volume:		10
Issue:		8
Page Number:		2559 - 2572
DOI:		10.1091/mbc.10.8.2559
ISSN/ISBN:		1059-1524 (Print) 1059-1524 (Linking)
Abstract:		"The mushroom-producing fungus Schizophyllum commune has thousands of mating types defined, in part, by numerous lipopeptide pheromones and their G protein-linked receptors. Compatible combinations of pheromones and receptors encoded by different mating types regulate a pathway of sexual development leading to mushroom formation and meiosis. A complex set of pheromone-receptor interactions maximizes the likelihood of outbreeding; for example, a single pheromone can activate more than one receptor and a single receptor can be activated by more than one pheromone. The current study demonstrates that the sex pheromones and receptors of Schizophyllum, when expressed in Saccharomyces cerevisiae, can substitute for endogenous pheromone and receptor and induce the yeast pheromone response pathway through the yeast G protein. Secretion of active Schizophyllum pheromone requires some, but not all, of the biosynthetic machinery used by the yeast lipopeptide pheromone a-factor. The specificity of interaction among pheromone-receptor pairs in Schizophyllum was reproduced in yeast, thus providing a powerful system for exploring molecular aspects of pheromone-receptor interactions for a class of seven-transmembrane-domain receptors common to a wide range of organisms"
Keywords:		ATP-Binding Cassette Transporters/genetics/metabolism Amino Acid Sequence Carboxy-Lyases/genetics/metabolism Fungal Proteins/*genetics/*metabolism GTP-Binding Proteins/metabolism *GTPase-Activating Proteins *Glycoproteins Mating Factor Membrane Proteins M;
Notes:		"MedlineFowler, T J DeSimone, S M Mitton, M F Kurjan, J Raper, C A eng Research Support, U.S. Gov't, Non-P.H.S. 1999/08/06 Mol Biol Cell. 1999 Aug; 10(8):2559-72. doi: 10.1091/mbc.10.8.2559"