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J Plant Physiol

Title:		Magnaporthe oryzae cell wall hydrolysate induces ROS and fungistatic VOCs in rice cell cultures
Author(s):		Forlani G; Occhipinti A; Bossi S; Bertea CM; Varese C; Maffei ME;
Address:		"Department of Biology and Evolution, University of Ferrara, via L. Borsari 46, I-44100 Ferrara, Italy"
Journal Title:		J Plant Physiol
Year:		2011
Volume:		20110809
Issue:		17
Page Number:		2041 - 2047
DOI:		10.1016/j.jplph.2011.06.014
ISSN/ISBN:		1618-1328 (Electronic) 0176-1617 (Linking)
Abstract:		"Plants react to microbial attack with a number of defense mechanisms, including the synthesis of volatile organic compounds (VOCs) and the production of reactive oxygen species (ROS). These responses are triggered by elicitors derived from either the cell surface of pathogens or the incomplete hydrolysis of the plant cell wall. Here we show the response of rice (Oryza sativa L., cv Gigante Vercelli) cell cultures following treatment with cell wall hydrolysates prepared from the rice blast Magnaporthe oryzae. Elicitation prompted the production of several plant VOCs, which were analyzed by stir bar sorptive extraction from both the liquid and head-space phase (SBSE and HSSE, respectively) and gas chromatography coupled to mass spectrometry (GC-MS) analysis. VOCs included alkanes, alkenes and long-chain alcohols as well as cinnamyl alcohol, myristicin, a sesquiterpene alcohol (caryolan-1-ol), 1-butanamide and 2-pentylfuran. The major released compounds, 1-octanol and 1-decanol, were found to induce ROS production in both elicited and non-elicited rice cells and showed fungistatic activity against the pathogen M. oryzae. The possible role of induced VOCs and ROS production in the plant-pathogen interaction is discussed"
Keywords:		1-Octanol/metabolism Cell Extracts/isolation & purification/*pharmacology Cell Wall/metabolism Fatty Alcohols/metabolism Host-Pathogen Interactions Magnaporthe/*chemistry/physiology Oryza/chemistry/*drug effects/microbiology/physiology Plant Diseases/micr;
Notes:		"MedlineForlani, Giuseppe Occhipinti, Andrea Bossi, Simone Bertea, Cinzia M Varese, Cristina Maffei, Massimo E eng Research Support, Non-U.S. Gov't Germany 2011/08/13 J Plant Physiol. 2011 Nov 15; 168(17):2041-7. doi: 10.1016/j.jplph.2011.06.014. Epub 2011 Aug 9"