| Title: | "Dynamics of volatilomes emitted during cross-talking of plant-growth-promoting bacteria and the phytopathogen, Fusarium solani" |
| Author(s): | Ana AG; Carrillo-Cerda HA; Rodriguez-Campos J; Velazquez-Fernandez JB; Patron-Soberano OA; Contreras-Ramos SM; |
| Address: | "Unidad de Tecnologia Ambiental, Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco A.C. (CIATEJ), Av. Normalistas No. 800, Col. Colinas de La Normal, 44270, Guadalajara, Jalisco, Mexico. Unidad de Servicios Analiticos Y Metrologicos (USAM), CIATEJ, Av. Normalistas No. 800, Col. Colinas de La Normal, 44270, Guadalajara, Jalisco, Mexico. jarodriguez@ciatej.mx. Catedra-Conacyt assigned to Unidad de Tecnologia Ambiental at CIATEJ, Guadalaja, Jalisco, Mexico. Division de Biologia Molecular, Instituto Potosino de Investigacion Cientifica Y Tecnologica A.C. (IPICYT), Camino a la Presa San Jose 2055, Lomas 4 feminine. Seccion, 78216, San Luis Potosi, San Luis Potosi, Mexico. Unidad de Tecnologia Ambiental, Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de Jalisco A.C. (CIATEJ), Av. Normalistas No. 800, Col. Colinas de La Normal, 44270, Guadalajara, Jalisco, Mexico. smcontreras@ciatej.mx" |
| Journal Title: | World J Microbiol Biotechnol |
| DOI: | 10.1007/s11274-020-02928-w |
| ISSN/ISBN: | 1573-0972 (Electronic) 0959-3993 (Linking) |
| Abstract: | "The dynamics of volatilomes emitted during the interaction between plant-growth-promoting bacteria (PGPB) and the phytopathogen Fusarium solani were evaluated for 5 days. The first screening was done to evaluate the antagonist activity of volatile compounds emitted by PGPB against F. solani. Volatilomes from 11 PGPB were determined individually and together with F. solani by using solid-phase microextraction coupled to gas-chromatography-mass spectrometry. Isolates of PGPB belonged to the Bacillus genus and inhibited from 18 to 24% the fungal mycelium growth. The isolates also induced morphological alterations of fungal hyphae, like small globular vesicles and the formation of chlamydospores, suggesting a stress mechanism response by the fungus. Volatilome profile showed 49 different compounds that appeared in the bacterial-fungal interaction, such as ketones, sesquiterpenes, monoterpenoids, alkanes, alkenes, carboxylic acids, and fatty acids. Some ketones and alcohols were detected in high abundance only in the interaction PGPB-fungus at 3 and 5 days. Bacillus circulans A19, Bacillus amyloliquefaciens A21, and Bacillus wiedmannii S18 shared a group of emitted alcohols and ketones when they were exposed to F. solani. F. solani produced its own volatilome profile, with the presence of sesquiterpenes, such as alpha-cubebene and caryophyllene, which increased significantly in co-incubation with the tested bacteria, suggesting chemical communication between them" |
| Keywords: | Alkanes/pharmacology Alkenes/pharmacology Antifungal Agents/chemistry/*pharmacology Bacillus Bacillus amyloliquefaciens Bacteria/drug effects/*metabolism *Bacterial Physiological Phenomena Carboxylic Acids/pharmacology Fatty Acids/pharmacology Fusarium/*d; |
| Notes: | "MedlineAna, A Gutierrez-Santa Carrillo-Cerda, H A Rodriguez-Campos, J Velazquez-Fernandez, J B Patron-Soberano, O A Contreras-Ramos, S M eng 292399/de Fomento Regional para el Desarrollo Cientifico, Tecnologico y de Innovacion/ Germany 2020/09/15 World J Microbiol Biotechnol. 2020 Sep 14; 36(10):152. doi: 10.1007/s11274-020-02928-w" |
