| Title: | "Analysis of yeast prp20 mutations and functional complementation by the human homologue RCC1, a protein involved in the control of chromosome condensation" |
| Author(s): | Fleischmann M; Clark MW; Forrester W; Wickens M; Nishimoto T; Aebi M; |
| Address: | "Institut fur Molekularbiologie I, Universitat Zurich, Switzerland" |
| ISSN/ISBN: | 0026-8925 (Print) 0026-8925 (Linking) |
| Abstract: | "Mutations in the PRP20 gene of yeast show a pleiotropic phenotype, in which both mRNA metabolism and nuclear structure are affected. srm1 mutants, defective in the same gene, influence the signal transduction pathway for the pheromone response. The yeast PRP20/SRM1 protein is highly homologous to the RCC1 protein of man, hamster and frog. In mammalian cells, this protein is a negative regulator for initiation of chromosome condensation. We report the analysis of two, independently isolated, recessive temperature-sensitive prp20 mutants. They have identical G to A transitions, leading to the alteration of a highly conserved glycine residue to glutamic acid. By immunofluorescence microscopy the PRP20 protein was localized in the nucleus. Expression of the RCC1 protein can complement the temperature-sensitive phenotype of prp20 mutants, demonstrating the functional similarity of the yeast and mammalian proteins" |
| Keywords: | "Alleles Blotting, Western *Cell Cycle Proteins *Chromosomes, Fungal *Chromosomes, Human DNA-Binding Proteins/*genetics Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Genes, Fungal Genetic Complementation Test *Guanine Nucleotide Exchan;" |
| Notes: | "MedlineFleischmann, M Clark, M W Forrester, W Wickens, M Nishimoto, T Aebi, M eng GM00521/GM/NIGMS NIH HHS/ GM31892/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Germany 1991/07/01 Mol Gen Genet. 1991 Jul; 227(3):417-23. doi: 10.1007/BF00273932" |
