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Metabolites

Title:		A GABA Receptor Modulator and Semiochemical Compounds Evidenced Using Volatolomics as Candidate Markers of Chronic Exposure to Fipronil in Apis mellifera
Author(s):		Fernandes V; Hidalgo K; Diogon M; Mercier F; Angenieux M; Ratel J; Delbac F; Engel E; Bouchard P;
Address:		"CNRS, UMR6023, Laboratoire Microorganismes Genome et Environnement, Universite Clermont Auvergne, F-63000 Clermont-Ferrand, France. INRAE, UR370 Qualite des Produites Animaux, MASS Group, F-63122 Saint-Genes-Champanelle, France"
Journal Title:		Metabolites
Year:		2023
Volume:		20230126
Issue:		2
Page Number:		-
DOI:		10.3390/metabo13020185
ISSN/ISBN:		2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking)
Abstract:		"Among the various 'omics' approaches that can be used in toxicology, volatolomics is in full development. A volatolomic study was carried out on soil bacteria to validate the proof of concept, and this approach was implemented in a new model organism: the honeybee Apis mellifera. Emerging bees raised in the laboratory in pain-type cages were used. Volatolomics analysis was performed on cuticles, fat bodies, and adhering tissues (abdomens without the digestive tract), after 14 and 21 days of chronic exposure to 0.5 and 1 microg/L of fipronil, corresponding to sublethal doses. The VOCs analysis was processed using an HS-SPME/GC-MS method. A total of 281 features were extracted and tentatively identified. No significant effect of fipronil on the volatolome could be observed after 14 days of chronic exposure. Mainly after 21 days of exposure, a volatolome deviation appeared. The study of this deviation highlighted 11 VOCs whose signal abundances evolved during the experiment. Interestingly, the volatolomics approach revealed a VOC (2,6-dimethylcyclohexanol) that could act on GABA receptor activity (the fipronil target) and VOCs associated with semiochemical activities (pheromones, repellent agents, and compounds related to the Nasonov gland) leading to a potential impact on bee behavior"
Keywords:		GABA modulator honeybees pesticide volatile organic compounds volatolomics;
Notes:		"PubMed-not-MEDLINEFernandes, Vincent Hidalgo, Kevin Diogon, Marie Mercier, Frederic Angenieux, Magaly Ratel, Jeremy Delbac, Frederic Engel, Erwan Bouchard, Philippe eng BEELOSS/Agence Nationale de la Recherche/ SYMBIOSE/Contrat de Plan Etat-Region/ Switzerland 2023/02/26 Metabolites. 2023 Jan 26; 13(2):185. doi: 10.3390/metabo13020185"