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Eur J Biochem

Title:		Ligand specificity of pheromone-binding proteins of the processionary moth
Author(s):		Feixas J; Prestwich GD; Guerrero A;
Address:		"Department of Biological Organic Chemistry, Consejo Superior de Investigaciones Cientificas, Barcelona, Spain"
Journal Title:		Eur J Biochem
Year:		1995
Volume:		234
Issue:		2
Page Number:		521 - 526
DOI:		10.1111/j.1432-1033.1995.521_b.x
ISSN/ISBN:		0014-2956 (Print) 0014-2956 (Linking)
Abstract:		"Photoaffinity labeling of proteins extracted from sensory hairs and antennal branches of the processionary moth Thaumetopoea pityocampa with a tritium-labeled diazoacetate analogue of the sex pheromone (Z)-13-hexadecen-11-ynyl acetate revealed a 15-kDa pheromone-binding protein in male moth sensory hairs (SH-15). A different 15-kDa protein in male antennal branches (B-15) was not photolabeled. All extracts except male sensory hairs showed a photolabeled 20-kDa protein; a photolabeled male 30-kDa protein in the branches (B-30) was also observed. The 20-kDa proteins in the sensory hairs (SH-20) and branches (B-20) showed differing affinities for the photoaffinity analogues; moreover, SH-15 exhibits higher affinity for the natural pheromone, (Z)-13-hexadecen-11-ynyl acetate, than for its alcohol metabolite and other analogues in competitive displacement experiments. The affinity shown by the pheromone-binding protein for the metabolic product suggests that the alcohol may be also transported by the binding protein. Interestingly, a shift in labeling from SH-15 to SH-20 was produced in the presence of an excess of the natural pheromone, its alcohol and other analogues. The binding showed little discrimination among structurally similar analogues of the pheromone, while saturated and aromatic molecules showed little affinity for the proteins of either sensory hairs or antennal branches"
Keywords:		Affinity Labels/metabolism Animals Carrier Proteins/*metabolism Female Lepidoptera/*metabolism Male Molecular Weight Pheromones/*metabolism Sensitivity and Specificity;
Notes:		"MedlineFeixas, J Prestwich, G D Guerrero, A eng Research Support, Non-U.S. Gov't England 1995/12/01 Eur J Biochem. 1995 Dec 1; 234(2):521-6. doi: 10.1111/j.1432-1033.1995.521_b.x"