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PLoS One

Title:		Diet-induced obesity in male C57BL/6 mice decreases fertility as a consequence of disrupted blood-testis barrier
Author(s):		Fan Y; Liu Y; Xue K; Gu G; Fan W; Xu Y; Ding Z;
Address:		"Department of Human Anatomy, Histology and Embryology, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory for Reproductive Medicine, Shanghai, China. Department of Medical Laboratory Science, The Central Hospital of Zhabei District, Shanghai, China. Department of Human Anatomy, Histology and Embryology, School of Medicine, Shanghai Jiao Tong University, Shanghai Key Laboratory for Reproductive Medicine, Shanghai, China; Department of Human Reproductive Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China"
Journal Title:		PLoS One
Year:		2015
Volume:		20150417
Issue:		4
Page Number:		e0120775 -
DOI:		10.1371/journal.pone.0120775
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"Obesity is a complex metabolic disease that is a serious detriment to both children and adult health, which induces a variety of diseases, such as cardiovascular disease, type II diabetes, hypertension and cancer. Although adverse effects of obesity on female reproduction or oocyte development have been well recognized, its harmfulness to male fertility is still unclear because of reported conflicting results. The aim of this study was to determine whether diet-induced obesity impairs male fertility and furthermore to uncover its underlying mechanisms. Thus, male C57BL/6 mice fed a high-fat diet (HFD) for 10 weeks served as a model of diet-induced obesity. The results clearly show that the percentage of sperm motility and progressive motility significantly decreased, whereas the proportion of teratozoospermia dramatically increased in HFD mice compared to those in normal diet fed controls. Besides, the sperm acrosome reaction fell accompanied by a decline in testosterone level and an increase in estradiol level in the HFD group. This alteration of sperm function parameters strongly indicated that the fertility of HFD mice was indeed impaired, which was also validated by a low pregnancy rate in their mated normal female. Moreover, testicular morphological analyses revealed that seminiferous epithelia were severely atrophic, and cell adhesions between spermatogenic cells and Sertoli cells were loosely arranged in HFD mice. Meanwhile, the integrity of the blood-testis barrier was severely interrupted consistent with declines in the tight junction related proteins, occludin, ZO-1 and androgen receptor, but instead endocytic vesicle-associated protein, clathrin rose. Taken together, obesity can impair male fertility through declines in the sperm function parameters, sex hormone level, whereas during spermatogenesis damage to the blood-testis barrier (BTB) integrity may be one of the crucial underlying factors accounting for this change"
Keywords:		"Animals Blood-Testis Barrier/*pathology Body Weight Clathrin/metabolism *Diet, High-Fat Estradiol/analysis Female Fertility/*physiology Lipids/blood Male Mice Mice, Inbred C57BL Models, Animal Obesity/*etiology Occludin/metabolism Receptors, Androgen/meta;"
Notes:		"MedlineFan, Yong Liu, Yue Xue, Ke Gu, Guobao Fan, Weimin Xu, Yali Ding, Zhide eng Research Support, Non-U.S. Gov't 2015/04/18 PLoS One. 2015 Apr 17; 10(4):e0120775. doi: 10.1371/journal.pone.0120775. eCollection 2015"