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In Vitro Cell Dev Biol Anim

Title:		Effects of mimosine on Wolbachia in mosquito cells: cell cycle suppression reduces bacterial abundance
Author(s):		Fallon AM;
Address:		"Department of Entomology, University of Minnesota, 1980 Folwell Ave, St. Paul, MN, 55108, USA. fallo002@umn.edu"
Journal Title:		In Vitro Cell Dev Biol Anim
Year:		2015
Volume:		20150528
Issue:		9
Page Number:		958 - 963
DOI:		10.1007/s11626-015-9918-7
ISSN/ISBN:		1543-706X (Electronic) 1071-2690 (Print) 1071-2690 (Linking)
Abstract:		"The plant allelochemical L-mimosine (beta-[N-(3-hydroxy-4-pyridone)]-alpha-aminopropionic acid; leucenol) resembles the nonessential amino acid, tyrosine. Because the obligate intracellular alphaproteobacterium, Wolbachia pipientis, metabolizes amino acids derived from host cells, the effects of mimosine on infected and uninfected mosquito cells were investigated. The EC50 for mimosine was 6-7 muM with Aedes albopictus C7-10 and C/wStr cell lines, and was not influenced by infection status. Mosquito cells responded to concentrations of mimosine substantially lower than those used to synchronize the mammalian cell cycle; at concentrations of 30-35 muM, mimosine reversibly arrested the mosquito cell cycle at the G1/S boundary and inhibited growth of Wolbachia strain wStr. Although lower concentrations of mimosine slightly increased wStr abundance, concentrations that suppressed the cell cycle reduced Wolbachia levels"
Keywords:		"Aedes/*cytology/*drug effects/microbiology Animals Cell Cycle/drug effects Cell Line/drug effects/microbiology Dose-Response Relationship, Drug Mimosine/*pharmacology Pheromones/pharmacology Wolbachia/*drug effects/growth & development/pathogenicity Aedes;"
Notes:		"MedlineFallon, Ann M eng R01 AI081322/AI/NIAID NIH HHS/ AI 081322/AI/NIAID NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Germany 2015/05/29 In Vitro Cell Dev Biol Anim. 2015 Oct; 51(9):958-63. doi: 10.1007/s11626-015-9918-7. Epub 2015 May 28"