| Title: | "Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis" |
| Author(s): | Evangelista M; Blundell K; Longtine MS; Chow CJ; Adames N; Pringle JR; Peter M; Boone C; |
| Address: | "Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada" |
| DOI: | 10.1126/science.276.5309.118 |
| ISSN/ISBN: | 0036-8075 (Print) 0036-8075 (Linking) |
| Abstract: | "The Saccharomyces cerevisiae BNI1 gene product (Bni1p) is a member of the formin family of proteins, which participate in cell polarization, cytokinesis, and vertebrate limb formation. During mating pheromone response, bni1 mutants showed defects both in polarized morphogenesis and in reorganization of the underlying actin cytoskeleton. In two-hybrid experiments, Bni1p formed complexes with the activated form of the Rho-related guanosine triphosphatase Cdc42p, with actin, and with two actin-associated proteins, profilin and Bud6p (Aip3p). Both Bni1p and Bud6p (like Cdc42p and actin) localized to the tips of mating projections. Bni1p may function as a Cdc42p target that links the pheromone response pathway to the actin cytoskeleton" |
| Keywords: | Actins/*metabolism Cell Cycle Proteins/*metabolism *Contractile Proteins Cytoskeleton/*metabolism Fungal Proteins/genetics/*metabolism GTP-Binding Proteins/*metabolism Microfilament Proteins/metabolism Morphogenesis Mutagenesis Profilins Recombinant Fusio; |
| Notes: | "MedlineEvangelista, M Blundell, K Longtine, M S Chow, C J Adames, N Pringle, J R Peter, M Boone, C eng GM31006/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1997/04/04 Science. 1997 Apr 4; 276(5309):118-22. doi: 10.1126/science.276.5309.118" |
