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Plant J

Title:		The MYB5-driven MBW complex recruits a WRKY factor to enhance the expression of targets involved in vacuolar hyper-acidification and trafficking in grapevine
Author(s):		Amato A; Cavallini E; Walker AR; Pezzotti M; Bliek M; Quattrocchio F; Koes R; Ruperti B; Bertini E; Zenoni S; Tornielli GB;
Address:		"Department of Biotechnology, University of Verona, Verona, Italy. CSIRO Agriculture & Food, Waite Campus, Urrbrae, South Australia, Australia. Swammerdam Institute of Life Sciences, University of Amsterdam, 1090GE, Amsterdam, the Netherlands. Department of Agriculture, Food, Natural Resources, Animals and Environment, University of Padova, Padova, Italy"
Journal Title:		Plant J
Year:		2019
Volume:		20190627
Issue:		6
Page Number:		1220 - 1241
DOI:		10.1111/tpj.14419
ISSN/ISBN:		1365-313X (Electronic) 0960-7412 (Linking)
Abstract:		"The accumulation of secondary metabolites and the regulation of tissue acidity contribute to the important traits of grape berry and influence plant performance in response to abiotic and biotic factors. In several plant species a highly conserved MYB-bHLH-WD (MBW) transcriptional regulatory complex controls flavonoid pigment synthesis and transport, and vacuolar acidification in epidermal cells. An additional component, represented by a WRKY-type transcription factor, physically interacts with the complex increasing the expression of some target genes and adding specificity for other targets. Here we investigated the function of MBW(W) complexes involving two MYBs (VvMYB5a and VvMYB5b) and the WRKY factor VvWRKY26 in grapevine (Vitis vinifera L.). Using transgenic grapevine plants we showed that these complexes affected different aspects of morphology, plant development, pH regulation, and pigment accumulation. Transcriptomic analysis identified a core set of putative target genes controlled by VvMYB5a, VvMYB5b, and VvWRKY26 in different tissues. Our data indicated that VvWRKY26 enhances the expression of selected target genes induced by VvMYB5a/b. Among these targets are genes involved in vacuolar hyper-acidification, such as the P-type ATPases VvPH5 and VvPH1, and trafficking, and genes involved in the biosynthesis of flavonoids. In addition, VvWRKY26 is recruited specifically by VvMYB5a, reflecting the functional diversification of VvMYB5a and VvMYB5b. The expression of MBWW complexes in vegetative organs, such as leaves, indicates a possible function of vacuolar hyper-acidification in the repulsion of herbivores and/or in developmental processes, as shown by defects in transgenic grape plants where the complex is inactivated"
Keywords:		"Anthocyanins/metabolism Biological Transport Flavonoids/metabolism Gene Expression Gene Expression Profiling Gene Expression Regulation, Plant/genetics P-type ATPases/genetics/*metabolism Petunia/genetics/metabolism Phenotype Plant Leaves/cytology/genetic;"
Notes:		"MedlineAmato, Alessandra Cavallini, Erika Walker, Amanda R Pezzotti, Mario Bliek, Mattijs Quattrocchio, Francesca Koes, Ronald Ruperti, Benedetto Bertini, Edoardo Zenoni, Sara Tornielli, Giovanni B eng Research Support, Non-U.S. Gov't England 2019/05/28 Plant J. 2019 Sep; 99(6):1220-1241. doi: 10.1111/tpj.14419. Epub 2019 Jun 27"