| Title: | Non-conventional gas phase remediation of volatile halogenated compounds by dehydrated bacteria |
| Author(s): | Erable B; Goubet I; Seltana A; Maugard T; |
| Address: | "UMR 6250 CNRS-ULR, LIENSs, Equipe BIotechnologie ENvironnementale, Batiment Marie Curie, Universite de La Rochelle, La Rochelle Cedex 1, France" |
| DOI: | 10.1016/j.jenvman.2009.01.010 |
| ISSN/ISBN: | 1095-8630 (Electronic) 0301-4797 (Linking) |
| Abstract: | "Traditional biological removal processes are limited by the low solubility of halogenated compounds in aqueous media. A new technology appears very suitable for the remediation of these volatile organic compounds (VOCs). Solid/gas bio-catalysis applied in VOC remediation can transform halogenated compounds directly in the gas phase using dehydrated cells as a bio-catalyst. The hydrolysis of volatile halogenated substrates into the corresponding alcohol was studied in a solid/gas biofilter where lyophilised bacterial cultures were used as the catalyst. Four strains containing dehalogenase enzymes were tested for the hydrolysis of 1-chlorobutane. The highest removal yield was obtained using the dhaA-containing strains, the maximal reaction rate of 0.8 micromol min(-1)g(-1) being observed with Escherichia coli BL21(DE3)(dhaA). Various treatments such as cell disruption by lysozyme or alkaline gas addition in the bio-filter could stabilise the dehalogenase activity of the bacteria. A pre-treatment of the dehydrated bacterial cells by ammonia vapour improved the stability of the catalyst and a removal activity of 0.9 micromol min(-1)g(-1) was then obtained for 60h. Finally, the process was extended to a range of halogenated substrates including bromo- and chloro-substrates. It was shown that the removal capacity for long halogenated compounds (C(5)-C(6)) was greatly increased relative to traditional biological processes" |
| Keywords: | "Air Pollutants/*metabolism Bacteria/*metabolism *Biodegradation, Environmental Volatile Organic Compounds/*metabolism;" |
| Notes: | "MedlineErable, Benjamin Goubet, Isabelle Seltana, Amira Maugard, Thierry eng Research Support, Non-U.S. Gov't England 2009/02/25 J Environ Manage. 2009 Jun; 90(8):2841-4. doi: 10.1016/j.jenvman.2009.01.010. Epub 2009 Feb 23" |
