| Title: | Both activation and repression of a-mating-type-specific genes in yeast require transcription factor Mcm1 |
| Address: | "Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853" |
| ISSN/ISBN: | 0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking) |
| Abstract: | "Mcm1 is a yeast transcription factor with homologs throughout the metazoa. MCM1 was first identified as a gene involved in maintenance of artificial minichromosomes in yeast. More recently Mcm1 has been shown to serve as a transcriptional regulator of mating-type-specific genes. Biochemical data suggest that Mcm1 coactivates alpha-specific genes and corepresses a-specific genes by binding to a 10-base-pair dyad symmetry element in their upstream regions. We reported previously that an mcm1 point mutation reduced activation of alpha-specific genes but had little effect on the expression of a-specific genes. We now show that another mcm1 allele, which depletes the Mcm1 protein, affects both activation and repression of a-specific genes. The mutant strain remains capable of high levels of pheromone induction of a-specific genes, although with retarded kinetics. Mcm1 joins an increasing number of transcription factors involved in both positive and negative regulation of gene expression" |
| Keywords: | "Alleles Chromosome Deletion Fungal Proteins/*genetics/metabolism *Gene Expression Regulation, Fungal *Genes, Fungal *Genes, Mating Type, Fungal Kinetics Mating Factor Minichromosome Maintenance 1 Protein Mutagenesis, Insertional Peptides/*genetics Pheromo;" |
| Notes: | "MedlineElble, R Tye, B K eng GM34190/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1991/12/01 Proc Natl Acad Sci U S A. 1991 Dec 1; 88(23):10966-70. doi: 10.1073/pnas.88.23.10966" |
