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Environ Microbiol

Title:		"Isolation of isoprene degrading bacteria from soils, development of isoA gene probes and identification of the active isoprene-degrading soil community using DNA-stable isotope probing"
Author(s):		El Khawand M; Crombie AT; Johnston A; Vavlline DV; McAuliffe JC; Latone JA; Primak YA; Lee SK; Whited GM; McGenity TJ; Murrell JC;
Address:		"University of East Anglia, Norwich Research Park, Norwich, UK. DuPont Industrial Biosciences, 925 Page Mill Road, Palo Alto, CA, 94304, USA. University of Essex, Wivenhoe Park, Colchester, UK"
Journal Title:		Environ Microbiol
Year:		2016
Volume:		20160630
Issue:		8
Page Number:		2743 - 2753
DOI:		10.1111/1462-2920.13345
ISSN/ISBN:		1462-2920 (Electronic) 1462-2912 (Linking)
Abstract:		"Emissions of biogenic volatile organic compounds (bVOCs), are an important element in the global carbon cycle, accounting for a significant proportion of fixed carbon. They contribute directly and indirectly to global warming and climate change and have a major effect on atmospheric chemistry. Plants emit isoprene to the atmosphere in similar quantities to emissions of methane from all sources and each accounts for approximately one third of total VOCs. Although methanotrophs, capable of growth on methane, have been intensively studied, we know little of isoprene biodegradation. Here, we report the isolation of two isoprene-degrading strains from the terrestrial environment and describe the design and testing of polymerase chain reaction (PCR) primers targeting isoA, the gene encoding the active-site component of the conserved isoprene monooxygenase, which are capable of retrieving isoA sequences from isoprene-enriched environmental samples. Stable isotope probing experiments, using biosynthesized (13) C-labelled isoprene, identified the active isoprene-degrading bacteria in soil. This study identifies novel isoprene-degrading strains using both culture-dependent and, for the first time, culture-independent methods and provides the tools and foundations for continued investigation of the biogeography and molecular ecology of isoprene-degrading bacteria"
Keywords:		"Atmosphere/analysis Bacteria/genetics/*metabolism Base Sequence Butadienes/*metabolism Carbon Cycle/*physiology Climate Change DNA/metabolism DNA Probes DNA, Bacterial/genetics Genome, Bacterial/genetics Hemiterpenes/*metabolism Methane/metabolism Oxygena;"
Notes:		"MedlineEl Khawand, Myriam Crombie, Andrew T Johnston, Antonia Vavlline, Dmitrii V McAuliffe, Joseph C Latone, Jacob A Primak, Yuliya A Lee, Sang-Kyu Whited, Gregg M McGenity, Terry J Murrell, J Colin eng England 2016/04/23 Environ Microbiol. 2016 Sep; 18(8):2743-53. doi: 10.1111/1462-2920.13345. Epub 2016 Jun 30"