| Title: | Impact of ion-pairs for the determination of multiclass antimicrobials residues in honey by LC-MS/MS |
| Author(s): | El Hawari K; Daher Z; Verdon E; Al Iskandarani M; |
| Address: | "a CNRSL, Lebanese Atomic Energy Commission (LAEC) , Laboratory for Analysis of Organic Compound (LAOC) , Beirut , Lebanon. b ANSES, French Agency for Safety of Food, Environmental and Occupational Health , Laboratory of Fougeres, European Union Reference Laboratory for Residues of Antimicrobial Veterinary Medicinal Products in Food from Animal Origin , Javene , Fougeres , France. c Faculty of Public Health I , Lebanese University , Hadath, Beirut , Lebanon" |
| Journal Title: | Food Addit Contam Part A Chem Anal Control Expo Risk Assess |
| DOI: | 10.1080/19440049.2017.1372641 |
| ISSN/ISBN: | 1944-0057 (Electronic) 1944-0057 (Linking) |
| Abstract: | "This study examines the behaviour of volatile perfluorinated carboxylic acids used as ion-pairing reagents for the separation of multiclass antimicrobial residues in honey (tetracyclines, sulphonamides, macrolides, and aminoglycosides). Heptafluorobutanoic acid and pentafluoropentanoic acid were compared as mobile phase additives at concentrations of 0-20 mmol.L(-1) using reversed-phase chromatography. Our objective was to find optimal conditions that enable the separation of antimicrobials with a minimum loss in sensitivity and efficiency. The influence of ion pairing on chromatographic performance was examined. Results showed that heptafluorobutanoic acid was able to separate aminoglycosides faster and more efficiently than pentafluoropentanoic acid, but at low concentrations of heptafluorobutanoic acid between 2.5 and 5 mM, tetracyclines showed poor chromatographic efficiency. A concentration of 10 mM was found to be optimal for all studied veterinary drugs. The method was then applied to determine multiclass antimicrobial residues in honey. Based on validation data, the mean recoveries of analytes ranged between 93% and 104%, and the intermediate precisions were <21%. The decision limit (CCalpha) and detection capability (CCbeta) were in the ranges of 5-25 and 7-33 mug kg(-1), respectively. The method was found to be suitable for use in analysis of veterinary drugs with a broader group of compounds found in food-producing animals" |
| Keywords: | "Anti-Infective Agents/*analysis Carboxylic Acids/*analysis Chromatography, Liquid Drug Residues/*analysis Fluorocarbons/*analysis Honey/*analysis Tandem Mass Spectrometry Antimicrobial Lc-ms/ms honey ion-pairing reagents perfluorinated carboxylic acids;" |
| Notes: | "MedlineEl Hawari, Khaled Daher, Zeina Verdon, Eric Al Iskandarani, Mohamad eng England 2017/09/05 Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2017 Dec; 34(12):2131-2143. doi: 10.1080/19440049.2017.1372641. Epub 2017 Sep 15" |
