| Title: | Comprehensive two-dimensional gas chromatography and three-dimensional fluorometry for detection of volatile and bioactive substances in some berries |
| Author(s): | Dymerski T; Namiesnik J; Vearasilp K; Arancibia-Avila P; Toledo F; Weisz M; Katrich E; Gorinstein S; |
| Address: | "Department of Analytical Chemistry, Chemical Faculty, Gdansk University of Technology, Gdansk 80 952, Poland. Electronic address: tomasz.dymerski@gmail.com. Department of Analytical Chemistry, Chemical Faculty, Gdansk University of Technology, Gdansk 80 952, Poland. Faculty of Pharmacy, Srinakharinwirot University, Bangkok, Thailand. Department of Basic Sciences, Universidad del Bio-Bio, P.O. Box 447, 3780000 Chillan, Chile. The Institute for Drug Research, School of Pharmacy, The Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel. The Institute for Drug Research, School of Pharmacy, The Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel. Electronic address: shela.gorin@mail.huji.ac.il" |
| DOI: | 10.1016/j.talanta.2014.11.061 |
| ISSN/ISBN: | 1873-3573 (Electronic) 0039-9140 (Linking) |
| Abstract: | "The volatile fractions of Cape gooseberry and blueberry were determined by headspace solid-phase microextraction coupled with comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (HS-SPME/GCxGC-TOFMS). The highest amount of alcohol (51.8%), ester (32.8%) and carboxylic acid (6.9%) was in blueberry in comparison with gooseberry and oppositely ketones (14.7%), aldehydes (9.9%) and terpenes (8%) were found in gooseberry. The bioactive compounds and antioxidant capacities were higher in blueberries than in gooseberries. Three dimensional fluorescence emission spectrometry (3D-FL) was applied to determine and to compare experimentally found binding parameters of berries extracts with human serum albumin (HSA). The fluorescence quenching of HSA by polyphenols from berries was a result of the formation of a polyphenol-HSA complex. The binding abilities of berries were highly correlated with the bioactivity of polyphenols and volatile substances. The cluster analysis (CA) and linear discriminant analysis (LDA) was applied to differentiate the berries samples according to their type" |
| Keywords: | Blueberry Plants Fluorometry Fruit/*chemistry Gas Chromatography-Mass Spectrometry Polyphenols/chemistry Ribes Serum Albumin/chemistry Volatile Organic Compounds/*analysis/chemistry Bioactivity Three-dimensional fluorescence spectroscopy Two-dimensional g; |
| Notes: | "MedlineDymerski, Tomasz Namiesnik, Jacek Vearasilp, Kann Arancibia-Avila, Patricia Toledo, Fernando Weisz, Moshe Katrich, Elena Gorinstein, Shela eng Netherlands 2015/01/27 Talanta. 2015 Mar; 134:460-467. doi: 10.1016/j.talanta.2014.11.061. Epub 2014 Dec 2" |
