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Plant Sci

Title:		Novel culture chamber to evaluate in vitro plant-microbe volatile interactions: Effects of Trichoderma harzianum volatiles on wheat plantlets
Author(s):		Alvarez-Garcia S; Manga-Robles A; Encina A; Gutierrez S; Casquero PA;
Address:		"Grupo Universitario de Investigacion en Ingenieria y Agricultura Sostenible (GUIIAS), Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad, Universidad de Leon, Avenida Portugal 41, 24071 Leon, Spain. Electronic address: salvg@unileon.es. Area de Fisiologia Vegetal, Dpto. Ingenieria y Ciencias Agrarias. Facultad de Ciencias Biologicas y Ambientales, Universidad de Leon, E-24071 Leon, Spain. Electronic address: amanr@unileon.es. Area de Fisiologia Vegetal, Dpto. Ingenieria y Ciencias Agrarias. Facultad de Ciencias Biologicas y Ambientales, Universidad de Leon, E-24071 Leon, Spain. Electronic address: a.encina@unileon.es. Grupo Universitario de Investigacion en Ingenieria y Agricultura Sostenible (GUIIAS), Area de Microbiologia, Escuela de Ingenieria Agraria y Forestal, Universidad de Leon, Campus de Ponferrada, Avenida Astorga s/n, 24401 Ponferrada, Spain. Electronic address: s.gutierrez@unileon.es. Grupo Universitario de Investigacion en Ingenieria y Agricultura Sostenible (GUIIAS), Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad, Universidad de Leon, Avenida Portugal 41, 24071 Leon, Spain. Electronic address: pacasl@unileon.es"
Journal Title:		Plant Sci
Year:		2022
Volume:		20220413
Issue:
Page Number:		111286 -
DOI:		10.1016/j.plantsci.2022.111286
ISSN/ISBN:		1873-2259 (Electronic) 0168-9452 (Linking)
Abstract:		"The field of plant-microbe interactions mediated by Biogenic Volatile Organic Compounds (BVOCs) still faces several limitations due to the lack of reliable equipment. We present a novel device designed to evaluate in vitro plant-microbe volatile interactions, the plant-microbe VOC Chamber. It was tested by evaluating the effects exerted on wheat development by volatiles from three Trichoderma harzianum strains, a wild type and two genetically modified strains; one expressing the tri5 gene, which leads to the synthesis and emission of the volatile trichodiene, and the other by silencing the erg1 gene, impairing ergosterol production. The wild type and the erg1-silenced strain enhanced fresh weight and length of the aerial part, but reduced root dry weight. Interestingly, no differences were found between them. Conversely, the tri5-transformant strain reduced root and aerial growth compared to the control and the other strains. No differences were observed regarding chlorophyll fluorescence quantum yield and leaf chlorophyll content, suggesting that the released BVOCs do not interfere with photosynthesis. The plant-microbe VOC Chamber proved to be a simple and reliable method to evaluate the in vitro effects of microbial BVOCs on plant development, perfect for the screening of microorganisms with interesting volatile traits"
Keywords:		Chlorophyll Hypocreales Plants *Triticum *Volatile Organic Compounds/pharmacology Bvoc Plant-microbe VOC Chamber Trichoderma Trichodiene Volatile Organic Compounds Wheat;
Notes:		"MedlineAlvarez-Garcia, Samuel Manga-Robles, Alba Encina, Antonio Gutierrez, Santiago Casquero, Pedro A eng Ireland 2022/06/02 Plant Sci. 2022 Jul; 320:111286. doi: 10.1016/j.plantsci.2022.111286. Epub 2022 Apr 13"