| Title: | "Cloning, overexpression, purification, crystallization and preliminary X-ray analysis of a female-specific lipocalin (FLP) expressed in the lacrimal glands of Syrian hamsters" |
| Author(s): | Dubey VP; Pal B; Srikantan S; Pottabathini S; De PK; Sankaranarayanan R; |
| Address: | "Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Uppal Road, Hyderabad 500 007, India" |
| Journal Title: | Acta Crystallogr Sect F Struct Biol Cryst Commun |
| DOI: | 10.1107/S1744309110008237 |
| ISSN/ISBN: | 1744-3091 (Electronic) 1744-3091 (Linking) |
| Abstract: | "Proteins belonging to the lipocalin superfamily are usually secretory proteins of molecular mass approximately 20 kDa with a hydrophobic pocket for the binding and transport of diverse small ligands. Various lipocalins have been associated with many biological processes, e.g. immunomodulation, odorant transport, pheromonal activity, retinoid transport, cancer-cell interactions etc. However, the exact functions of many lipocalins and the ligands bound by them are unclear. Previously, the cDNA of a 20 kDa lipocalin (FLP) which is female-specifically expressed in the lacrimal glands of Syrian (golden) hamsters and secreted in the tears of females has been identified and cloned. His-tagged recombinant FLP (rFLP) has now been cloned, overexpressed in Escherichia coli as a soluble protein and purified to homogeneity using Ni-affinity followed by size-exclusion chromatography. Purified rFLP was crystallized using the sitting-drop vapour-diffusion method. The crystals tested belonged to space group P2(1)2(1)2(1) and diffracted to beyond 1.86 A resolution. Solvent-content analysis indicated the presence of one monomer in the asymmetric unit" |
| Keywords: | "Animals Cloning, Molecular Cricetinae Crystallization Crystallography, X-Ray Female Gene Expression Lacrimal Apparatus/*chemistry Lipocalins/*chemistry/genetics/isolation & purification *Mesocricetus;" |
| Notes: | "MedlineDubey, Ved Prakash Pal, Biswajit Srikantan, Subramanya Pottabathini, Sambhavi De, Prabir Kumar Sankaranarayanan, Rajan eng England 2010/05/07 Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1; 66(Pt 5):509-12. doi: 10.1107/S1744309110008237. Epub 2010 Apr 29" |
