| Title: | Methodology for exposing avian embryos to quantified levels of airborne aromatic compounds associated with crude oil spills |
| Author(s): | Dubansky B; Verbeck G; Mach P; Burggren W; |
| Address: | "University of North Texas, Department of Biological Sciences, Developmental Integrative Biology Cluster, 1155 Union Circle, Denton, TX, 76203, United States. Electronic address: Benjamin.Dubansky@unt.edu. University of North Texas, Department of Chemistry and Biochemistry, Laboratory for Imaging Mass Spectrometry, 1417 Hickory Street, Denton, TX, 76203, United States. University of North Texas, Department of Chemistry and Biochemistry, Laboratory for Imaging Mass Spectrometry, 1417 Hickory Street, Denton, TX, 76203, United States; Aberdeen Proving Ground, 5183 Balckhawk Rd, E3150, Gunpowder, MD, 21010, United States. University of North Texas, Department of Biological Sciences, Developmental Integrative Biology Cluster, 1155 Union Circle, Denton, TX, 76203, United States" |
| Journal Title: | Environ Toxicol Pharmacol |
| DOI: | 10.1016/j.etap.2018.01.005 |
| ISSN/ISBN: | 1872-7077 (Electronic) 1382-6689 (Linking) |
| Abstract: | "Oil spills on birds and other organisms have focused primarily on direct effects of oil exposure through ingestion or direct body fouling. Little is known of indirect effects of airborne volatiles from spilled oil, especially on vulnerable developing embryos within the bird egg. Here a technique is described for exposing bird embryos in the egg to quantifiable amounts of airborne volatile toxicants from Deepwater Horizon crude oil. A novel membrane inlet mass spectrometry system was used to measure major classes of airborne oil-derived toxicants and correlate these exposures with biological endpoints. Exposure induced a reduction in platelet number and increase in osmolality of the blood of embryos of the chicken (Gallus gallus). Additionally, expression of cytochrome P4501A, a protein biomarker of oil exposure, occurred in renal, pulmonary, hepatic and vascular tissues. These data confirm that this system for generating and measuring airborne volatiles can be used for future in-depth analysis of the toxicity of volatile organic compounds in birds and potentially other terrestrial organisms" |
| Keywords: | Air Pollutants/*toxicity Animals Biomarkers/metabolism Chick Embryo/*drug effects/metabolism Chickens Cytochrome P-450 CYP1A1/metabolism Hematocrit Kidney/drug effects/embryology/metabolism Liver/drug effects/embryology/metabolism Lung/drug effects/embryo; |
| Notes: | "MedlineDubansky, Benjamin Verbeck, Guido Mach, Phillip Burggren, Warren eng Netherlands 2018/02/07 Environ Toxicol Pharmacol. 2018 Mar; 58:163-169. doi: 10.1016/j.etap.2018.01.005. Epub 2018 Jan 10" |
