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Clin Chim Acta

Title:		A novel non-invasive colorectal cancer diagnostic method: Volatile organic compounds as biomarkers
Author(s):		Alustiza M; Ripoll L; Canals A; Murcia O; Martinez-Roca A; Garcia-Heredia A; Giner-Calabuig M; Jover R; Vidal L;
Address:		"Laboratorio de Investigacion, Hospital General Universitario Dr. Balmis, Instituto de Investigacion Sanitaria y Biomedica de Alicante (ISABIAL), Alicante, Spain. Departamento de Quimica Analitica, Nutricion y Bromatologia e Instituto Universitario de Materiales, Universidad de Alicante, P.O. Box 99, 03080 Alicante, Spain. Laboratorio de Investigacion, Hospital General Universitario Dr. Balmis, Instituto de Investigacion Sanitaria y Biomedica de Alicante (ISABIAL), Alicante, Spain; Servicio de Medicina Digestiva, Hospital General Universitario Dr. Balmis, Instituto de Investigacion Sanitaria y Biomedica de Alicante (ISABIAL), Alicante, Spain. Laboratorio de Investigacion, Hospital General Universitario Dr. Balmis, Instituto de Investigacion Sanitaria y Biomedica de Alicante (ISABIAL), Alicante, Spain; Servicio de Medicina Digestiva, Hospital General Universitario Dr. Balmis, Instituto de Investigacion Sanitaria y Biomedica de Alicante (ISABIAL), Alicante, Spain. Electronic address: Jover_rod@gva.es. Laboratorio de Investigacion, Hospital General Universitario Dr. Balmis, Instituto de Investigacion Sanitaria y Biomedica de Alicante (ISABIAL), Alicante, Spain; Departamento de Quimica Analitica, Nutricion y Bromatologia e Instituto Universitario de Materiales, Universidad de Alicante, P.O. Box 99, 03080 Alicante, Spain. Electronic address: lorena.vidal@ua.es"
Journal Title:		Clin Chim Acta
Year:		2023
Volume:		20230301
Issue:
Page Number:		117273 -
DOI:		10.1016/j.cca.2023.117273
ISSN/ISBN:		1873-3492 (Electronic) 0009-8981 (Linking)
Abstract:		"INTRODUCTION: Population-based fecal tests for colorectal cancer (CRC) screening have shown to reduce mortality thanks to the early detection of the disease. However, currently available fecal tests are limited in their sensitivity and specificity. Our aim is to look for volatile organic compounds in fecal samples as biomarkers for CRC detection. MATERIAL AND METHODS: Eighty participants were included; 24 had adenocarcinoma, 24 had adenomatous polyps and 32 presented no neoplasms. Fecal samples were collected 48 h preceding the colonoscopy from all participants, except CRC patient samples that were collected after 3-4 weeks from the colonoscopy. Magnetic headspace adsorptive extraction (Mag-HSAE) followed by thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS) was performed on stool samples to identify volatile organic compounds as biomarkers. RESULTS: p-Cresol was significantly more abundant in the cancer samples (P < 0.001) with an area under the curve (AUC) of 0.85 (CI 95%; 0.737-0.953), having a sensitivity and specificity of 83% and 82%, respectively. In addition, 3(4H)-dibenzofuranone,4a,9b-dihydro-8,9b-dimethyl- (3(4H)-DBZ) was also more abundant in the cancer samples (P < 0.001) with an AUC of 0.77 (CI 95%; 0.635-0.905), sensitivity of 78% and specificity of 75%. When combined (p-cresol and 3(4H)-DBZ), the AUC was 0.86, sensitivity 87% and specificity 79%. p-Cresol also appeared to be promising as a biomarker for pre-malignant lesions with an AUC of 0.69 (CI 95%; 0.534-0.862), sensitivity 83% and specificity 63%, P = 0.045. CONCLUSIONS: Volatile organic compounds emitted from feces and determined by a sensitive analytical methodology (Mag-HSAE-TD-GC-MS), employing a magnetic graphene oxide as extractant phase, could be used as a potential screening technology for CRC and pre-malignant lesions"
Keywords:		"Humans *Volatile Organic Compounds/analysis Biomarkers, Tumor/analysis Cresols *Colorectal Neoplasms/diagnosis/pathology Sensitivity and Specificity Early Detection of Cancer/methods Feces/chemistry Cancer diagnostic Colorectal cancer Diagnostic tests Lar;"
Notes:		"MedlineAlustiza, Miren Ripoll, Laura Canals, Antonio Murcia, Oscar Martinez-Roca, Alejandro Garcia-Heredia, Anabel Giner-Calabuig, Mar Jover, Rodrigo Vidal, Lorena eng Netherlands 2023/03/03 Clin Chim Acta. 2023 Mar 1; 542:117273. doi: 10.1016/j.cca.2023.117273. Epub 2023 Mar 1"