| Title: | Breathing Rhythm Variations during Wash-In Do Not Influence Exhaled Volatile Organic Compound Profile Analyzed by an Electronic Nose |
| Author(s): | Dragonieri S; Quaranta VN; Carratu P; Ranieri T; Buonamico E; Carpagnano GE; |
| Address: | "Respiratory Diseases, University of Bari, 70121 Bari, Italy. Pulmonology, Di Venere Hospital, 70131 Bari, Italy. Internal Medicine 'A. Murri', University of Bari, 70121 Bari, Italy" |
| DOI: | 10.3390/molecules26092695 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "E-noses are innovative tools used for exhaled volatile organic compound (VOC) analysis, which have shown their potential in several diseases. Before obtaining a full validation of these instruments in clinical settings, a number of methodological issues still have to be established. We aimed to assess whether variations in breathing rhythm during wash-in with VOC-filtered air before exhaled air collection reflect changes in the exhaled VOC profile when analyzed by an e-nose (Cyranose 320). We enrolled 20 normal subjects and randomly collected their exhaled breath at three different breathing rhythms during wash-in: (a) normal rhythm (respiratory rate (RR) between 12 and 18/min), (b) fast rhythm (RR > 25/min) and (c) slow rhythm (RR < 10/min). Exhaled breath was collected by a previously validated method (Dragonieri et al., J. Bras. Pneumol. 2016) and analyzed by the e-nose. Using principal component analysis (PCA), no significant variations in the exhaled VOC profile were shown among the three breathing rhythms. Subsequent linear discriminant analysis (LDA) confirmed the above findings, with a cross-validated accuracy of 45% (p = ns). We concluded that the exhaled VOC profile, analyzed by an e-nose, is not influenced by variations in breathing rhythm during wash-in" |
| Keywords: | Adult Analysis of Variance Breath Tests/*methods *Electronic Nose Exhalation/*physiology Female Humans Male Principal Component Analysis Volatile Organic Compounds/*analysis breath analysis breathing rhythm e-nose electronic nose volatile organic compound; |
| Notes: | "MedlineDragonieri, Silvano Quaranta, Vitaliano Nicola Carratu, Pierluigi Ranieri, Teresa Buonamico, Enrico Carpagnano, Giovanna Elisiana eng Switzerland 2021/06/03 Molecules. 2021 May 4; 26(9):2695. doi: 10.3390/molecules26092695" |
