| Title: | Antennal transcriptome analysis of odorant-binding proteins and characterization of GOBP2 in the variegated cutworm Peridroma saucia |
| Author(s): | Dong JF; Wang K; Sun YL; Tian CH; Wang SL; |
| Address: | "College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang, China. State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou, China" |
| DOI: | 10.3389/fphys.2023.1241324 |
| ISSN/ISBN: | 1664-042X (Print) 1664-042X (Electronic) 1664-042X (Linking) |
| Abstract: | "Odorant-binding proteins (OBPs) are expressed at extremely high concentrations in the chemo-sensilla lymph of insects and have long been thought to be crucial for delivering the semiochemicals to the odorant receptors. They are represented by multiple classes: general odorant-binding proteins (GOBP1 and GOBP2) and pheromone-binding proteins. In the current study, we identified a total of 35 OBPs in the antennal transcriptome of Peridroma saucia, a worldwide pest that causes serious damage to various crops. A gene expression value (TPM, transcripts per million) analysis revealed that seven OBPs (PsauPBP1/2/3, PsauGOBP1/2, PsauOBP6, and PsauOBP8) were highly abundant in the antennae. Next, we focused on the expression and functional characterization of PsauGOBP2. Real-time quantitative-PCR analysis demonstrated that PsauGOBP2 was predominantly expressed in the antennae of both sexes. Fluorescence binding assays showed that the recombinant PsauGOBP2 strongly binds to the female sex pheromone components Z11-16: Ac (K(i) = 4.2 muM) and Z9-14: Ac (K(i) = 4.9 muM) and binds moderately (6 microM |
| Keywords: | antennal transcriptome fluorescence binding assay general odorant-binding protein molecular docking tissue expression; |
| Notes: | "PubMed-not-MEDLINEDong, Jun-Feng Wang, Ke Sun, Ya-Lan Tian, Cai-Hong Wang, Shao-Li eng Switzerland 2023/08/28 Front Physiol. 2023 Aug 10; 14:1241324. doi: 10.3389/fphys.2023.1241324. eCollection 2023" |
