Bedoukian   RussellIPM   RussellIPM   Piezoelectric Micro-Sprayer


Home
Animal Taxa
Plant Taxa
Semiochemicals
Floral Compounds
Semiochemical Detail
Semiochemicals & Taxa
Synthesis
Control
Invasive spp.
References

Abstract

Guide

Alphascents
Pherobio
InsectScience
E-Econex
Counterpart-Semiochemicals
Print
Email to a Friend
Kindly Donate for The Pherobase

« Previous Abstract"Commentary on effects of anthropogenic and natural organic chemicals on development, swimming behavior, and reproduction of Daphnia, a key member of aquatic ecosystems"    Next AbstractInvestigation of the phytochemical composition and antioxidant properties of chinar (Platanus orientalis L.) leaf infusion against ethanol-induced oxidative stress in rats »

Biochem J


Title:Glycosyl-phosphatidylinositol anchor attachment in a yeast in vitro system
Author(s):Doering TL; Schekman R;
Address:"Department of Molecular and Cell Biology, Barker Hall, Howard Hughes Research Institute, University of California, Berkeley, CA 94720-3202, USA"
Journal Title:Biochem J
Year:1997
Volume:328 ( Pt 2)
Issue:Pt 2
Page Number:669 - 675
DOI: 10.1042/bj3280669
ISSN/ISBN:0264-6021 (Print) 1470-8728 (Electronic) 0264-6021 (Linking)
Abstract:"The yeast mating pheromone precursor prepro-alpha factor was fused to C-terminal signals for glycosyl-phosphatidylinositol (GPI) anchor attachment, based on the sequence of the Saccharomyces cerevisiae protein Gas1p. Maturation of fusion proteins expressed in vivo required the presence of both a functional GPI attachment site and the synthesis of GPI precursors. Constructs were translated in vitro for use in cell-free studies of glycolipid attachment. The radiolabelled polypeptides were post-translationally translocated into yeast microsomes, where at least one third of the molecules received a GPI anchor. This approach offers distinct advantages over anchor attachment reactions that require co-translational translocation of secretory peptide substrates"
Keywords:Biological Transport Cell-Free System Fungal Proteins/genetics/*metabolism Glycosylphosphatidylinositols/*metabolism Membrane Glycoproteins/genetics/*metabolism Protein Precursors/genetics/*metabolism Recombinant Fusion Proteins/metabolism Research Design;
Notes:"MedlineDoering, T L Schekman, R eng Research Support, Non-U.S. Gov't England 1998/01/24 Biochem J. 1997 Dec 1; 328 ( Pt 2)(Pt 2):669-75. doi: 10.1042/bj3280669"

 
Back to top
 
Citation: El-Sayed AM 2024. The Pherobase: Database of Pheromones and Semiochemicals. <http://www.pherobase.com>.
© 2003-2024 The Pherobase - Extensive Database of Pheromones and Semiochemicals. Ashraf M. El-Sayed.
Page created on 27-11-2024