Title: | Drying protocol does not alter plant delta(13)C and delta(15)N: a baseline survey for ecological studies |
Author(s): | Diaz-Alvarez EA; de la Barrera E; |
Address: | "Instituto de Investigaciones Forestales, Universidad Veracruzana, Xalapa, Mexico. Instituto de Investigaciones en Ecosistemas y Sustentabilidad, Universidad Nacional Autonoma de Mexico, Morelia, Mexico" |
Journal Title: | Isotopes Environ Health Stud |
DOI: | 10.1080/10256016.2019.1673747 |
ISSN/ISBN: | 1477-2639 (Electronic) 1025-6016 (Linking) |
Abstract: | "Drying protocols might alter the isotopic signatures of plant samples given that high temperatures can volatilize various organic compounds or delay the halting of physiological processes at lower drying temperatures. We thus evaluated the effect of four drying protocols on the carbon and nitrogen isotopic signatures for 23 species of plants. In particular, leaves were either freeze-dried, placed in a herbarium drying stove (ca. 50 degrees C), in a gravity convection oven (80 degrees C), or microwaved (900 W) in 2-minute pulses, until constant weight. For each species, neither treatment led to significantly different delta(13)C values, which ranged from -31.7 to -12.4 per thousand. The delta(15)N values of 21 of the species considered were not affected by the drying protocols, ranging from -11.6 to -8.8 per thousand. For Tillandsia makoyana, significant differences were observed between the freeze dried and the microwaved samples and between the freeze dried samples and those dried at 50 degrees C for Macroptilium gibbosifolium" |
Keywords: | Carbon Isotopes/*analysis Desiccation/*methods Environmental Monitoring/*methods Fabaceae/chemistry *Freeze Drying Hot Temperature Nitrogen Isotopes/*analysis Plant Leaves/*chemistry Surveys and Questionnaires Volatilization Atmospheric pollution biomonit; |
Notes: | "MedlineDiaz-Alvarez, Edison A de la Barrera, Erick eng England 2019/10/11 Isotopes Environ Health Stud. 2019 Dec; 55(6):526-531. doi: 10.1080/10256016.2019.1673747. Epub 2019 Oct 9" |