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Phytochemistry

Title:		Metabolomics in melon: a new opportunity for aroma analysis
Author(s):		Allwood JW; Cheung W; Xu Y; Mumm R; de Vos RC; Deborde C; Biais B; Maucourt M; Berger Y; Schaffer AA; Rolin D; Moing A; Hall RD; Goodacre R;
Address:		"School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK; School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. Electronic address: j.w.allwood@bham.ac.uk. School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK. Plant Research International, P.O. Box 16, 6700 AA Wageningen, Netherlands; Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CC Leiden, Netherlands. Plant Research International, P.O. Box 16, 6700 AA Wageningen, Netherlands; Netherlands Metabolomics Centre, Einsteinweg 55, 2333 CC Leiden, Netherlands; Centre for BioSystems Genomics, P.O. Box 98, 6700AB Wageningen, Netherlands. INRA, UMR1332 Biologie du Fruit et Pathologie, INRA - Universite de Bordeaux, Centre INRA de Bordeaux, IBVM, CS20032, F-33140 Villenave d'Ornon, France; Metabolome Facility of Bordeaux Functional Genomics Centre, Centre INRA de Bordeaux, IBVM, F-33140 Villenave d'Ornon, France. INRA, UMR1332 Biologie du Fruit et Pathologie, INRA - Universite de Bordeaux, Centre INRA de Bordeaux, IBVM, CS20032, F-33140 Villenave d'Ornon, France. Metabolome Facility of Bordeaux Functional Genomics Centre, Centre INRA de Bordeaux, IBVM, F-33140 Villenave d'Ornon, France; Universite de Bordeaux, UMR1332 Biologie du Fruit et Pathologie, INRA - Universite de Bordeaux, Centre INRA de Bordeaux, IBVM, CS20032, F-33140 Villenave d'Ornon, France. Agricultural Research Organisation (ARO), The Volcani Center, Bet Dagan 50250, Israel. School of Chemistry, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK; Manchester Centre for Integrative Systems Biology, Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK"
Journal Title:		Phytochemistry
Year:		2014
Volume:		20140110
Issue:
Page Number:		61 - 72
DOI:		10.1016/j.phytochem.2013.12.010
ISSN/ISBN:		1873-3700 (Electronic) 0031-9422 (Print) 0031-9422 (Linking)
Abstract:		"Cucumis melo fruit is highly valued for its sweet and refreshing flesh, however the flavour and value are also highly influenced by aroma as dictated by volatile organic compounds (VOCs). A simple and robust method of sampling VOCs on polydimethylsiloxane (PDMS) has been developed. Contrasting cultivars of C. melo subspecies melo were investigated at commercial maturity: three cultivars of var. Cantalupensis group Charentais (cv. Cezanne, Escrito, and Dalton) known to exhibit differences in ripening behaviour and shelf-life, as well as one cultivar of var. Cantalupensis group Ha'Ogan (cv. Noy Yisre'el) and one non-climacteric cultivar of var. Inodorus (cv. Tam Dew). The melon cultivar selection was based upon fruits exhibiting clear differences (cv. Noy Yisre'el and Tam Dew) and similarities (cv. Cezanne, Escrito, and Dalton) in flavour. In total, 58 VOCs were detected by thermal desorption (TD)-GC-MS which permitted the discrimination of each cultivar via Principal component analysis (PCA). PCA indicated a reduction in VOCs in the non-climacteric cv. Tam Dew compared to the four Cantalupensis cultivars. Within the group Charentais melons, the differences between the short, mid and long shelf-life cultivars were considerable. (1)H NMR analysis led to the quantification of 12 core amino acids, their levels were 3-10-fold greater in the Charentais melons, although they were reduced in the highly fragrant cv. Cezanne, indicating their role as VOC precursors. This study along with comparisons to more traditional labour intensive solid phase micro-extraction (SPME) GC-MS VOC profiling data has indicated that the high-throughput PDMS method is of great potential for the assessment of melon aroma and quality"
Keywords:		Cucumis melo/chemistry/*metabolism Fruit/chemistry/*metabolism *Metabolomics Odorants/*analysis Volatile Organic Compounds/*analysis/metabolism Amino acid Aroma Cucumis melo Gas chromatography mass spectrometry (GC-MS) Metabolomics Proton-nuclear magnetic;
Notes:		"MedlineAllwood, J William Cheung, William Xu, Yun Mumm, Roland De Vos, Ric C H Deborde, Catherine Biais, Benoit Maucourt, Mickael Berger, Yosef Schaffer, Arthur A Rolin, Dominique Moing, Annick Hall, Robert D Goodacre, Royston eng Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2014/01/15 Phytochemistry. 2014 Mar; 99:61-72. doi: 10.1016/j.phytochem.2013.12.010. Epub 2014 Jan 10"