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Appl Environ Microbiol


Title:Different fecal microbiotas and volatile organic compounds in treated and untreated children with celiac disease
Author(s):Di Cagno R; Rizzello CG; Gagliardi F; Ricciuti P; Ndagijimana M; Francavilla R; Guerzoni ME; Crecchio C; Gobbetti M; De Angelis M;
Address:"Department of Plant Protection and Applied Microbiology, University of Bari, Italy"
Journal Title:Appl Environ Microbiol
Year:2009
Volume:20090417
Issue:12
Page Number:3963 - 3971
DOI: 10.1128/AEM.02793-08
ISSN/ISBN:1098-5336 (Electronic) 0099-2240 (Print) 0099-2240 (Linking)
Abstract:"This study aimed at investigating the fecal microbiotas of children with celiac disease (CD) before (U-CD) and after (T-CD) they were fed a gluten-free diet and of healthy children (HC). Brothers or sisters of T-CD were enrolled as HC. Each group consisted of seven children. PCR-denaturing gradient gel electrophoresis (DGGE) analysis with V3 universal primers revealed a unique profile for each fecal sample. PCR-DGGE analysis with group- or genus-specific 16S rRNA gene primers showed that the Lactobacillus community of U-CD changed significantly, while the diversity of the Lactobacillus community of T-CD was quite comparable to that of HC. Compared to HC, the ratio of cultivable lactic acid bacteria and Bifidobacterium to Bacteroides and enterobacteria was lower in T-CD and even lower in U-CD. The percentages of strains identified as lactobacilli differed as follows: HC (ca. 38%) > T-CD (ca. 17%) > U-CD (ca. 10%). Lactobacillus brevis, Lactobacillus rossiae, and Lactobacillus pentosus were identified only in fecal samples from T-CD and HC. Lactobacillus fermentum, Lactobacillus delbrueckii subsp. bulgaricus, and Lactobacillus gasseri were identified only in several fecal samples from HC. Compared to HC, the composition of Bifidobacterium species of T-CD varied, and it varied even more for U-CD. Forty-seven volatile organic compounds (VOCs) belonging to different chemical classes were identified using gas-chromatography mass spectrometry-solid-phase microextraction analysis. The median concentrations varied markedly for HC, T-CD, and U-CD. Overall, the r(2) values for VOC data for brothers and sisters were equal to or lower than those for unrelated HC and T-CD. This study shows the effect of CD pathology on the fecal microbiotas of children"
Keywords:"Bacteria/*classification/genetics/*isolation & purification *Biodiversity Celiac Disease/*microbiology Child Cluster Analysis DNA Fingerprinting DNA, Bacterial/genetics DNA, Ribosomal/genetics Electrophoresis, Polyacrylamide Gel Feces/*chemistry/*microbio;"
Notes:"MedlineDi Cagno, Raffaella Rizzello, Carlo G Gagliardi, Francesca Ricciuti, Patrizia Ndagijimana, Maurice Francavilla, Ruggiero Guerzoni, M Elisabetta Crecchio, Carmine Gobbetti, Marco De Angelis, Maria eng Comparative Study 2009/04/21 Appl Environ Microbiol. 2009 Jun; 75(12):3963-71. doi: 10.1128/AEM.02793-08. Epub 2009 Apr 17"

 
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