| Title: | A direct quantitative analysis method for monitoring biogenic volatile organic compounds released from leaves of Pelargonium hortorum in situ |
| Author(s): | Deng X; Peng J; Luo B; Wei M; Hu W; Du J; |
| Address: | "Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Shanghai 200032, China. xjdeng@iris.sipp.ac.cn" |
| DOI: | 10.1007/s00216-004-2874-9 |
| ISSN/ISBN: | 1618-2642 (Print) 1618-2642 (Linking) |
| Abstract: | "A direct quantitative method is presented that is based upon the use of multiple headspace solid phase microextraction (HS-SPME) to monitor biogenic volatile organic compounds (BVOCs) released from a living leaf of Pelargonium hortorum in situ. Seventeen BVOCs were detected by GC-MS after a single SPME extraction using a CAR/DVB/PDMS fibre. An internal standard was employed to determine the absolute amounts of seven terpenoid compounds released from a P. hortorum leaf. The quantitative analysis was performed over two days, with extraction preformed for 20 min every 3 h. The amount of volatiles extracted varied with the time of day, with two maxima recorded at 14:00 (day 1) and 17:00 (day 2), corresponding to 236 and 277 ng of the seven terpenoids recorded, respectively. These results indicate that multiple HS-SPME in combination with an internal standard is a simple, quick, and quantitative technique for analysising BVOC emissions from a live plant sample" |
| Keywords: | "Calibration Chemistry Techniques, Analytical/methods Organic Chemicals/*analysis Pelargonium/*chemistry Plant Leaves/*chemistry Regression Analysis Solvents/chemistry Time Factors Volatilization;" |
| Notes: | "MedlineDeng, Xiaojun Peng, Jinyin Luo, Bin Wei, Ming Hu, Wenli Du, Jiawei eng Research Support, Non-U.S. Gov't Germany 2004/11/13 Anal Bioanal Chem. 2004 Dec; 380(7-8):950-7. doi: 10.1007/s00216-004-2874-9. Epub 2004 Nov 5" |
