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Front Vet Sci

Title:		NEFAs Influence the Inflammatory and Insulin Signaling Pathways Through TLR4 in Primary Calf Hepatocytes in vitro
Author(s):		Deng Q; Du L; Zhang Y; Liu G;
Address:		"College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China. Inner Mongolia Minzu University Key Laboratory for Prevention and Control of Herbivorous Livestock Perinatal Diseases, Tongliao, China. College of Veterinary Medicine, Jilin University, Changchun, China"
Journal Title:		Front Vet Sci
Year:		2021
Volume:		20211213
Issue:
Page Number:		755505 -
DOI:		10.3389/fvets.2021.755505
ISSN/ISBN:		2297-1769 (Print) 2297-1769 (Electronic) 2297-1769 (Linking)
Abstract:		"Transition dairy cows are often in a state of negative energy balance because of decreased dry matter intake and increased energy requirements, initiating lipid mobilization and leading to high serum beta-hydroxybutyrate (BHBA) and non-esterified fatty acid (NEFAs) levels, which can induce ketosis and fatty liver in dairy cows. Inflammation and insulin resistance are also common diseases in the perinatal period of dairy cows. What is the relationship between negative energy balance, insulin resistance and inflammation in dairy cows? To study the role of non-esterified fatty acids in the nuclear factor kappa beta (NF-kappaB) inflammatory and insulin signaling pathways through Toll-like receptor 4 (TLR4), we cultured primary calf hepatocytes and added different concentrations of NEFAs to assess the mRNA and protein levels of inflammatory and insulin signaling pathways. Our experiments indicated that NEFAs could activate the NF-kappaB inflammatory signaling pathway and influence insulin resistance through TLR4. However, an inhibitor of TLR4 alleviated the inhibitory effects of NEFAs on the insulin pathway. In conclusion, all of these results indicate that high-dose NEFAs (2.4 mM) can activate the TLR4/NF-kappaB inflammatory signaling pathway and reduce the sensitivity of the insulin pathway through the TLR4/PI3K/AKT metabolic axis"
Keywords:		NEFAs NF-kappaB signaling pathway bovine hepatocytes insulin resistance lipid metabolism;
Notes:		"PubMed-not-MEDLINEDeng, Qinghua Du, Liyin Zhang, Yuming Liu, Guowen eng Switzerland 2021/12/31 Front Vet Sci. 2021 Dec 13; 8:755505. doi: 10.3389/fvets.2021.755505. eCollection 2021"