| Title: | Metabolomics analysis of human sweat collected after moderate exercise |
| Author(s): | Delgado-Povedano MM; Calderon-Santiago M; Luque de Castro MD; Priego-Capote F; |
| Address: | "Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Cordoba, E-14071 Cordoba, Spain; ceiA3 Agroalimentary Excellence Campus, University of Cordoba, Cordoba, Spain; Maimonides Institute of Biomedical Research (IMIBIC), Reina Sofia University Hospital, Cordoba, Spain; CIBER Fragilidad y Envejecimiento Saludable (CIBERfes), Instituto de Salud Carlos III, Spain. Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Cordoba, E-14071 Cordoba, Spain; ceiA3 Agroalimentary Excellence Campus, University of Cordoba, Cordoba, Spain; Maimonides Institute of Biomedical Research (IMIBIC), Reina Sofia University Hospital, Cordoba, Spain; CIBER Fragilidad y Envejecimiento Saludable (CIBERfes), Instituto de Salud Carlos III, Spain. Electronic address: qa1lucam@uco.es. Department of Analytical Chemistry, Annex Marie Curie Building, Campus of Rabanales, University of Cordoba, E-14071 Cordoba, Spain; ceiA3 Agroalimentary Excellence Campus, University of Cordoba, Cordoba, Spain; Maimonides Institute of Biomedical Research (IMIBIC), Reina Sofia University Hospital, Cordoba, Spain; CIBER Fragilidad y Envejecimiento Saludable (CIBERfes), Instituto de Salud Carlos III, Spain. Electronic address: q72prcaf@uco.es" |
| DOI: | 10.1016/j.talanta.2017.09.028 |
| ISSN/ISBN: | 1873-3573 (Electronic) 0039-9140 (Linking) |
| Abstract: | "Sweat is a promising biofluid scarcely used in clinical analysis despite its non-invasive sampling. A more frequent clinical use of sweat requires to know its whole composition, especially concerning to non-polar compounds, and the development of analytical strategies for its characterization. The aim of the present study was to compare different sample preparation strategies to maximize the detection of metabolites in sweat from humans collected after practicing moderate exercise. Special emphasis was put on non-polar compounds as they have received scant attention in previous studies dealing with this biofluid. Sample preparation by liquid-liquid extraction (LLE) using extractants with different polarity index was compared to deproteination. Then, derivatization by methoxymation with subsequent silylation was compared to direct analysis of sweat extracts to check the influence of derivatization on the subsequent determination of volatile organic compounds (VOCs). 135 compounds were tentatively identified by combining spectral and retention time information after analysis by gas chromatography coupled to mass spectrometry in high resolution mode (GC-TOF/MS). Lipids, VOCs, benzenoids and other interesting metabolites such as alkaloids and ethanolamines were identified. Among the tested protocols, methyoxiamination plus silylation after LLE with dichloromethane was the best option to obtain a representative snapshot of sweat metabolome collected from different body parts after moderate exercise. Passive and active sweat pools from a cohort of volunteers (n = 6) were compared to detect compositional differences which can be explained by the sampling process and sweating induction. As most of the identified compounds are metabolites involved in key biochemical pathways, this study opens new opportunities to extend the applicability of human sweat as a source of metabolite biomarkers of pathologies or specific processes such as dehydration or nutritional unbalance" |
| Keywords: | *Exercise Humans Liquid-Liquid Extraction *Metabolomics Solvents/chemistry Sweat/chemistry/*metabolism Active sweat Exercise Gas chromatography-mass spectrometry Metabolomics Passive sweat Sample preparation; |
| Notes: | "MedlineDelgado-Povedano, M M Calderon-Santiago, M Luque de Castro, M D Priego-Capote, F eng Netherlands 2017/11/08 Talanta. 2018 Jan 15; 177:47-65. doi: 10.1016/j.talanta.2017.09.028. Epub 2017 Sep 15" |
