| Title: | "Involvement of OsRIP1, a ribosome-inactivating protein from rice, in plant defense against Nilaparvata lugens" |
| Author(s): | De Zaeytijd J; Chen P; Scheys F; Subramanyam K; Dubiel M; De Schutter K; Smagghe G; Van Damme EJ; |
| Address: | "Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000 Ghent, Belgium; Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000 Ghent, Belgium. Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000 Ghent, Belgium. Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000 Ghent, Belgium. Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, B-9000 Ghent, Belgium. Electronic address: ElsJM.VanDamme@UGent.be" |
| DOI: | 10.1016/j.phytochem.2019.112190 |
| ISSN/ISBN: | 1873-3700 (Electronic) 0031-9422 (Linking) |
| Abstract: | "Rice is the most important staple food in the world, but rice production is challenged by several biotic stress factors like viruses, bacteria, fungi and pest insects. One of the most notorious pest insects is Nilaparvata lugens, commonly known as the brown planthopper, which feeds on rice phloem sap and can cause serious damage to rice fields. In order to protect themselves, plants express a wide array of defense proteins such as ribosome-inactivating proteins (RIPs). This study shows that the expression of 'OsRIP1' is highly induced in rice plants infested with N. lugens, with transcript levels more than 100-fold upregulated in infested plants compared to non-infested plants. Furthermore, recombinant OsRIP1 was toxic for brown planthoppers when administered through liquid artificial diet. OsRIP1 inactivated insect ribosomes in vitro, suggesting that its toxicity relates to the enzymatic activity of OsRIP1. Over-expression of OsRIP1 in transgenic rice plants did not affect the performance of insects reared on these plants, most likely due to insufficient concentrations of OsRIP1 in the phloem. The data obtained in this research indicate that OsRIP1 can play a role in plant defense against herbivorous insects" |
| Keywords: | Animals Hemiptera/*drug effects Oryza/*chemistry Phytochemicals/chemistry/isolation & purification/*pharmacology Saporins/chemistry/isolation & purification/*metabolism Nilaparvata lugens Oryza sativa Plant defense Ribosome-inactivating protein; |
| Notes: | "MedlineDe Zaeytijd, Jeroen Chen, Pengyu Scheys, Freja Subramanyam, Kondeti Dubiel, Malgorzata De Schutter, Kristof Smagghe, Guy Van Damme, Els Jm eng England 2019/11/16 Phytochemistry. 2020 Feb; 170:112190. doi: 10.1016/j.phytochem.2019.112190. Epub 2019 Nov 12" |
