| Title: | The faecal metabolome and mycobiome in Parkinson's disease |
| Author(s): | De Pablo-Fernandez E; Gebeyehu GG; Flain L; Slater R; Frau A; Ijaz UZ; Warner T; Probert C; |
| Address: | "Reta Lila Weston Institute of Neurological Studies and Queen Square Brain Bank for Neurological Disorders, UCL Queen Square Institute of Neurology, 1 Wakefield Street, WC1N 1PJ, London, United Kingdom. Electronic address: eduardo.fernandez.13@ucl.ac.uk. Institute of Systems, Molecular and Integrative Biology, University of Liverpool, The Henry Wellcome Laboratory, Nuffield Building, Crown Street, L69 3GE, Liverpool, United Kingdom. Electronic address: hlggasha@doctors.org.uk. Institute of Systems, Molecular and Integrative Biology, University of Liverpool, The Henry Wellcome Laboratory, Nuffield Building, Crown Street, L69 3GE, Liverpool, United Kingdom. James Watt School of Engineering, University of Glasgow, Rankine Building, Oakfield Avenue, G12 8QQ, Glasgow, United Kingdom. Reta Lila Weston Institute of Neurological Studies and Queen Square Brain Bank for Neurological Disorders, UCL Queen Square Institute of Neurology, 1 Wakefield Street, WC1N 1PJ, London, United Kingdom" |
| Journal Title: | Parkinsonism Relat Disord |
| DOI: | 10.1016/j.parkreldis.2022.01.005 |
| ISSN/ISBN: | 1873-5126 (Electronic) 1353-8020 (Linking) |
| Abstract: | "BACKGROUND: Gut fungal composition and its metabolites have not been assessed simultaneously in Parkinson's disease (PD) despite their potential pathogenic contribution. OBJECTIVE: To evaluate the faecal metabolome and mycobiome in PD by assessing volatile organic compounds (VOCs) and fungal rRNA. METHODS: Faecal VOCs from 35 PD patients and two control groups (n = 35; n = 15) were assessed using gas chromatography and mass spectrometry. DNA was extracted from 44 samples: 18S rRNA gene amplicons were prepared and sequenced. Metabolomics, mycobiome and integrated analyses were performed. RESULTS: Several VOCs were more abundant and short chain fatty acids were less abundant in PD. Hanseniaspora, Kazachstania, uncultured Tremellaceae and Penicillium genera were more abundant, and Saccharomyces less abundant in PD (FDR<0.0007). Torulaspora was associated with PD and two VOCs. CONCLUSION: PD patients had a distinct metabolome and mycobiome suggesting that fungal dysbiosis may contribute to PD pathogenesis" |
| Keywords: | Gas Chromatography-Mass Spectrometry *Gastrointestinal Microbiome/genetics Humans Metabolome *Mycobiome *Parkinson Disease/metabolism Fungal dysbiosis Metabolomics Mycobiome Parkinson's disease Volatile organic compounds; |
| Notes: | "MedlineDe Pablo-Fernandez, Eduardo Gebeyehu, Gerum Gashaw Flain, Luke Slater, Rachael Frau, Alessandra Ijaz, Umer Zeeshan Warner, Thomas Probert, Chris eng MR/P023606/1/MRC_/Medical Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2022/01/20 Parkinsonism Relat Disord. 2022 Feb; 95:65-69. doi: 10.1016/j.parkreldis.2022.01.005. Epub 2022 Jan 13" |
