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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		Headspace solid phase microextraction/gas chromatography-mass spectrometry combined to chemometric analysis for volatile organic compounds determination in canine hair: a new tool to detect dog contamination by visceral leishmaniasis
Author(s):		de Oliveira LS; Rodrigues Fde M; de Oliveira FS; Mesquita PR; Leal DC; Alcantara AC; Souza BM; Franke CR; Pereira PA; de Andrade JB;
Address:		"Instituto de Quimica, Universidade Federal da Bahia, Campus Universitario de Ondina, 40170-290 Salvador, BA, Brazil"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2008
Volume:		20081002
Issue:		2
Page Number:		392 - 398
DOI:		10.1016/j.jchromb.2008.09.028
ISSN/ISBN:		1570-0232 (Print) 1570-0232 (Linking)
Abstract:		"A new analytical methodology using HS-SPME/GC-MS was optimized in order to attain maximum sensitivity, using multivariate strategies. The proposed method was employed to evaluate the VOC profile exhaled from canine hair samples collected from 8 healthy dogs and from 16 dogs infected by Leishmania infantum. 274 VOCs were detected, which could be identified as aldehydes, ketones and hydrocarbons. After application of the Soft Independent Modeling of Class Analogy (SIMCA) and Principal Component Analysis (PCA) healthy and infected dogs, with similar VOCs profiles, could be separately grouped, based on compounds such as 2-hexanone, benzaldehyde, and 2,4-nonadienal. The proposed method is non-invasive, painless, readily accepted by dog owners and could be useful to identify several biomarkers with applications in the diagnosis of diseases"
Keywords:		"Animals Biomarkers/analysis Case-Control Studies Dog Diseases/*diagnosis Dogs Gas Chromatography-Mass Spectrometry/*methods Hair/*chemistry Leishmaniasis, Visceral/diagnosis/*veterinary Linear Models Multivariate Analysis Principal Component Analysis Soli;"
Notes:		"Medlinede Oliveira, Lidia S Rodrigues, Frederico de M de Oliveira, Fabio S Mesquita, Paulo R R Leal, Danielle C Alcantara, Adriano C Souza, Barbara M Franke, Carlos R Pereira, Pedro A de P de Andrade, Jailson B eng Research Support, Non-U.S. Gov't Netherlands 2008/10/24 J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Nov 15; 875(2):392-8. doi: 10.1016/j.jchromb.2008.09.028. Epub 2008 Oct 2"